碘缺乏和甲状腺功能减退仔鼠海马CaMKⅡ表达的改变  

Effect of iodine deficiency and hypothyroidism on the protein expressions of CaMKⅡin the hippocampus of pups

作　　者：魏薇[1] 董静[1] 刘万洋[1] 王毅[1] 陈杰[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生与环境卫生学教研室,沈阳110001

出　　处：《卫生研究》2010年第2期180-183,共4页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.30500400)

摘　　要：目的观察多功能钙/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)在碘缺乏和甲状腺功能减退的大鼠仔鼠海马中蛋白表达的影响。方法健康2月龄雌性Wistar大鼠,交配妊娠后,取孕鼠28只,按体重随机分成对照组、甲状腺功能减退组和碘缺乏组,甲状腺功能减退组根据饮水中含丙基硫尿嘧啶(PTU)剂量分为5和15mg/L组,每组7只孕鼠。分别于出生后(PN)7、14、和21天时,每组随机取5只仔鼠,灌流固定大脑,进行组织病理切片和免疫组化染色,观察分析海马CaMKⅡ表达。结果对照组仔鼠海马CA1、CA3和DG区均呈强阳性染色,在神经元细胞胞浆内充满CaMKⅡ。5、15mg/L和碘缺乏组仔鼠海马免疫反应产物的分布与对照组一致,但与对照组相比染色强度逐渐降低。PN21和PN14时,仔鼠海马CA1、CA3和DG区平均积分光密度在碘缺乏组[PN21:(26.05±4.98)、(30.79±3.22)、(26.40±2.63);PN14:(25.48±4.87)、(44.17±5.91)、(26.41±3.01)]和15mg/L组[PN21:(17.02±2.68)、(24.57±6.62)、(20.18±4.05);PN14:(20.66±3.51)、(34.94±5.09)、(27.32±4.97)]明显低于对照组[PN21:(57.75±13.22)、(65.03±6.20)、(49.39±8.41),P<0.05;PN14:(54.08±12.00)、(71.04±5.07)、(78.52±12.42),P<0.05]。PN7时,碘缺乏组和15mg/L组仔鼠海马CA1区平均积分光密度在碘缺乏组(25.74±3.33)和15mg/L组(26.89±5.25)明显低于对照组(40.53±3.65),P<0.05。结论碘缺乏和甲状腺功能减退可下调海马组织CaMKⅡ蛋白表达。Objective To observe the effect of iodine deficiency and hypothyroidism on the protein expressions of CaMKⅡ in the hippocampus of pups. Methods Female Wistar rats （ n = 28） after pregnancy were randomly divided into control group,hypothyroid group and iodine deficient group. According to the dose of PTU in fed water, hypothyroid group was divided into 5 mg /L group and 15 mg /L group （ 7 in each ）. 5 pups from each group were sacrificed and perfused intracardially in PN7,PN14 and PN21. Brains were removed,fixed and sectioned coronally. All sections were observed and analyzed the protein exression of CaMK Ⅱ by immunohistochemistry in the hippocampus CA1,CA3 and DG regions. Results Control group in CA1,CA3 and DG regions of the hippocampus had strong positive staining and the cytoplasm of neurons were filled with CaMKⅡ. The distribution of the immune reaction product in hippocampus of 5mg / L,15mg / L and iodine groups were in line with the control group,but the staining intensity as compared with the control group gradually decreased. In PN21 and PN14,integrated optical density average of CaMKⅡ in CA1,CA3 and DG regions of the hippocampus in iodine deficient group [PN21： （26. 05 ± 4. 98）、（30. 79 ± 3. 22）、（26. 40 ± 2. 63）; PN14：（25. 48 ± 4. 87）、（44. 17 ± 5. 91）、（26. 41 ± 3. 01）] and 15 mg /L groups [PN21： （17. 02 ± 2. 68）、（24. 57 ± 6. 62）、（20. 18 ± 4. 05）; PN14：（20. 66 ± 3. 51）、（34. 94 ± 5. 09）、（27. 32 ± 4. 97）]were significantly lower than those of controls [PN21： （57. 75 ± 13. 22）、（65. 03 ± 6. 20）、（49. 39 ± 8. 41）,P 0. 05; PN14： （54. 08 ± 12. 00）、（71. 04 ± 5. 07）、（78. 52 ± 12. 42）,P 0. 05]. InPN7,integrated optical density average of CaMK Ⅱ in CA1 region of the hippocampus in iodine deficient group （25. 74 ± 3. 33） and 15 mg/L groups （26. 89 ± 5. 25） were significantly lower than those of controls（40. 53 ± 3. 65）,P 0. 05. Conclusion Iodine deficiency and hy

关 键 词：碘缺乏 甲状腺功能减退 海马 CaMKⅡ 

分 类 号：R994.6[医药卫生—毒理学]