家蚕丝氨酸蛋白酶抑制剂基因serpin-6在不同发育时期的表达分析  

The Expression Profile of Serine Protease Inhibitor Gene serpin-6 in Bombyx mori at Different Developmental Stages

作　　者：俞燕芳[1,2] 查宏贤[1,2] 王彦云[1,2] 卫正国[1,3,2] 李兵[1,3,2] 陈玉华[1,3,2] 许雅香[1,3,2] 沈卫德[1,3,2] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]现代丝绸国家工程实验室,江苏苏州215123 [3]苏州大学蚕桑研究所,江苏苏州215123

出　　处：《蚕业科学》2010年第2期341-345,共5页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(No.2005CB12-1005)

摘　　要：丝氨酸蛋白酶抑制剂(serpin)是一类具有重要生理调控功能的蛋白酶超家族。为研究家蚕serpin-6基因(Bmserpin-6)在蚕体内的功能,利用半定量RT-PCR方法,分析该基因mRNA在家蚕不同发育时期的转录水平。Bmserpin-6基因在家蚕各个发育阶段均有表达,在4龄眠期、5龄6d、吐丝48h(化蛹前)和化蛹6d的转录水平较高,在5龄4d、吐丝初期和蛹末期的转录水平较低。Bmserpin-6基因在5龄期家蚕精巢和卵巢中的表达均呈现先下降后上升的趋势。研究结果表明,Bmserpin-6基因mRNA的转录具有明显的时空特异性,推测其在家蚕不同的变态发育期执行不同的功能。Serine protease inhibitor （serpin） genes comprise a large gene family. Their protein products regulate a wide variety of proteinase-dependent physiological functions. In order to study the function of Bmserpin-6 gene in Bombyx mori, the transcriptional levels of Bmserpin-6 at different developmental stages were determined by semi-quantitative RT- PCR. The results revealed that the transcriptional level of Bmserpin-6 gene is different at different developmental stages. It was transcribed throughout all the developmental stages, from the newly hatched larva to the moth. The transcriptional levels of Bmserpin-6 gene in the 4th moulting larva, day 6 larva of the 5th instar, larva at 48 hour of the spinning stage （ before pupation）, and day 6 pupae were relatively high, while those in day 4 larva of the 5th instar, newly spinning larva, and day 13 pupae were relatively low. The transcriptional levels of Bmserpin-6 gene in testes and ovaries showed atendency of rise first and then fall. The results indicated that transcription of Bmserpin-6 is stage-specific. It was suggested that Bmserpin-6 plays different roles in Bombyx mori during different metamorphic stages.

关 键 词：家蚕 丝氨酸蛋白酶抑制剂 基因转录 变态发育 半定量RT—PCR 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]