高效液相色谱-荧光分析法同时测定人体尿液中黄蝶呤与异黄蝶呤  

Determination of Xanthopterin and Isoxanthopterin in Human Urine by High Performance Liquid Chromatography with Fluorescence Detection

作　　者：万益群[1] 陈林[1] 

机构地区：[1]南昌大学分析测试中心,江西南昌330047

出　　处：《分析科学学报》2010年第2期171-174,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20765002);江西省自然科学基金(No.0620049)

摘　　要：建立了高效液相色谱-荧光法同时测定癌症病人尿液中黄蝶呤及异黄蝶呤的新方法。选择荧光检测波长λex=345nm,λem=420nm。以磷酸盐缓冲溶液(pH=7.5)-甲醇(体积比为98∶2)为流动相,流速1.0mL/min,黄蝶呤与异黄蝶呤含量分别在0.0013～0.945μg/mL及0.00017～0.118μg/mL范围内与色谱峰面积呈良好的线性关系,线性相关系数分别为0.9999和0.9996,检出限分别为0.5ng/mL和0.05ng/mL,加标平均回收率在86.2%～107.5%之间。方法应用于癌症病人尿样分析,取得了较好的结果。The high performance liquid chromatography （HPLC） with fluorescence detection method was developed for the determination of xanthopterin and isoxanthopterin in human urine. The mobile phase was phosphate buffer（pH=7.5）-methanol （98：2,V/V） with a flow rate of 1.0 mL/min,and the excitation and emission wavelengths were 345 nm and 420 nm,respectively. The linear ranges of xanthopterin and isoxanthopterin were 0.0013 μg/mL～0.945 μg/mL and 0.00017 μg/mL～0.118 μg/mL with the linear regression equations of y=3×106x＋28959 （r=0.9999） and y=1×108x-162133 （r=0.9996）,respectively. The detection limits for xanthopterin and isoxanthopterin were 0.5 ng/mL and 0.05 ng/mL and the recoveries were in the range of 86.2%～107.5%. The proposed method was successfully applied to the determination of xanthopterin and isoxanthopterin in human urine.

关 键 词：黄蝶呤 异黄蝶呤 尿液 高效液相色谱 荧光检测 

分 类 号：O657.3[理学—分析化学]