两种去除血清高丰度蛋白方法的比较  

Comparison of Two Methods to Eliminate High-abundance Proteins in Human Serum

作　　者：覃健[1] 张志勇[1] 何敏[2] 胡天桥[3] 

机构地区：[1]广西医科大学公共卫生学院职业卫生与环境卫生教研室,广西南宁530021 [2]广西医科大学医学科学实验中心 [3]广西柳州市疾病预防控制中心

出　　处：《预防医学情报杂志》2010年第4期322-324,共3页Journal of Preventive Medicine Information

基　　金：广西自然科学基金资助项目(桂科攻0592007-1C;桂科能05112001-4;桂科能0630006-5E;桂科攻0993003C-13)

摘　　要：目的比较乙腈沉淀法和MERCK公司的血清白蛋白和IgG去除试剂盒去除人血清中高丰度蛋白的效果。方法应用乙腈沉淀法和MERCK公司的血清白蛋白和IgG去除试剂盒对血清样品进行处理,Tricine-SDS-PAGE电泳和SELDI-TOF-MS检测去除高丰度蛋白的效果。结果乙腈沉淀法能在去除血清高中丰度蛋白的同时收获更多的10kD以下的小分子量蛋白,MERCK公司的试剂盒也能有效地去除血清高中丰度蛋白,特异性较好。结论2种方法均能去除血清中高丰度蛋白,需根据研究目的选择适合的方法。Objectives To compare the effects of acetonitrile precipitation and Proteo Extract Albumin/IgG Removal Kit in eliminating high-abundance proteins in human serum. Methods Elimination of serum high-abundance proteins, such as albumin and IgG, were performed with aeetonitrile precipitation and ProteoExtract Albumin/IgG Removal Kit of Merck Company respectively. Results The acetonitrile precipitation could eliminate the vast;majority of high abundance proteins in serum and gain more proteins of low molecular weight. The kit could eliminate high abundant proteins with high specificity too. Conclusion The two methods could be used to eliminate high abundant proteins in human serum and could be chosen according to the purpose of research.

关 键 词：高丰度蛋白 去除 比较 

分 类 号：R446[医药卫生—诊断学]