青藤碱对脑缺血再灌注损伤大鼠环氧化酶-2表达和血脑屏障通透性的影响  

Effect of Sinomenine on the Expression of Cyclooxygenase-2 and the Permeability of the Blood-brain Barrier Following Cerebral Ischemic Reperfusion Injury in Rats

作　　者：俸军林[1] 李浩[1] 蒋静子[1] 吴岚[1] 刘开祥[1] 唐永刚[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541001

出　　处：《时珍国医国药》2010年第4期806-808,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然科学基金(No.0848015)

摘　　要：目的探讨青藤碱(sinomenine,Sin)对缺血再灌注损伤大鼠脑组织保护作用及对环氧化酶-2(cyclooxygenase-2,COX-2)表达和血脑屏障通透性的影响。方法将大鼠随机分为假手术组、缺血再灌注组、Sin低剂量治疗组和Sin高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。Sin低(Sin30 mg/kg)、高剂量(60 mg/kg)治疗组于术前30 min分别给予大鼠腹腔注射。用免疫组化法观察缺血90 min再灌注24 h大鼠额顶部皮质COX-2表达及脑含水量和伊文斯蓝(EB)含量变化,并进行2,3,5-三苯基氯化四氮唑(TTC)染色观察脑梗塞体积变化和神经症状评分。结果Sin高、低剂量治疗组神经功能缺失评分较缺血再灌注组降低,高、低剂量组之间差异亦具有统计学意义(P<0.05)。Sin高、低剂量治疗组脑梗塞体积较缺血再灌注组减小,高、低剂量组之间差异亦具有统计学意义(P<0.05)。与假手术组比较,缺血再灌注组额顶部皮质COX-2表达明显增加;与缺血再灌注组比较,Sin高、低剂量治疗组COX-2表达均显著减少(P<0.05),高、低剂量组之间差异亦具有统计学意义(P<0.05)。与假手术组比较,缺血再灌注组脑含水量和EB含量明显升高;与缺血再灌注组比较,Sin高、低剂量治疗组脑含水量和EB含量明显降低(P<0.05),高、低剂量组之间差异亦具有统计学意义(P<0.05)。结论Sin对缺血再灌注脑损伤具有保护作用,其机制可能是通过抑制再灌注损伤后COX-2表达,减轻血脑屏障通透性。Objective To study the the effect of sinomenine on expression of cyclooxygenase - 2 and the permeability of the blood - brain barrier of cerebral ischemia reperfusion（I/R） injury in rats. Methods In this experiment, rats were randomly divided into 4 groups：sham operated group, I/R group, low sinomenine treated group and high sinomenine treated group. The focal middle cerebral artery occlusion（MCAO） model was made by suture -occluded method. Sinomenine was given intraperitoneally to rats 30min before focal cerebral ischemia operation respectively . After 90min MCAO following 24h of reperfusion, the expression of eyclooxygenase - 2 with immunohistochemistry were investigated. Brain water content and the content of Evens blue （EB） were investigated . TTC staining and the neurological score were also investigated. Results Compared with IR group, the neurologieal score was decreased in low and high dose sinomenine treated groups （ all P 〈 0.05 ）. （ 2 ） Compared with I/R group, low and high sinomenine treated groups dose - dependently reduced cerebral infarction （ all P 〈 0.05 ）. （ 3 ） Compared with sham operated group , expression of cyelooxygenase -2 was increased at 24h reperfusion in the frontal and parietal cortex （P 〈 0.05 ）. Compared with I/R group, low and high sinomenine treated groups dose - dependently reduced expression of cyelooxygenase - 2 （ all P 〈 0. 05 ）. （4） Compared with sham operated group, brain water content and the content of EB were increased at 24h of reperfusion. Compared with I/R groups, low and high sinomenine treated group dose - dependently reduced brain water content and the content of EB （P 〈 0.05 ）. Conclusion Sinomenine may obviously reduce cerebral isehemia -reperfusion injury by deereasing the expression of cyclooxygenase - 2 and the permeability of blood - brain barrier . It has protective effect on cerebral ischemia injury.

关 键 词：青藤碱 缺血再灌注 环氧化酶-2 血脑屏障 

分 类 号：R962[医药卫生—药理学]