辣椒细胞质雄性不育系及其保持系的AFLP分析  

AFLP Analysis of Cytoplasmic Male-sterile Line and Its Maintainer Line of Pepper

作　　者：罗向东[1,2] 戴亮芳[1,2] 陈劲枫[2] 王述彬[3] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江西师范大学生命科学学院,江西南昌330022 [3]江苏省农业科学院蔬菜研究所,江苏南京210014

出　　处：《安徽农业科学》2010年第10期4999-5001,共3页Journal of Anhui Agricultural Sciences

基　　金："十一五"国家"863"计划项目(2006AA100108-3-2);国家自然科学基金项目(30860120;30900781);江西省教育厅科技计划项目(GJJ08159);校青年成长基金项目(2398)

摘　　要：[目的]对辣椒细胞质雄性不育系及其保持系进行AFLP分析,为进一步深入研究辣椒细胞质雄性不育的分子机制奠定基础。[方法]采用AFLP技术对辣椒细胞质雄性不育系21A及其保持系21B进行分析,获得不育系21A特异扩增片段。将特异扩增片段回收测序并用BLASTn和BLASTX程序在NCBI数据库中进行同源性检索和相似性比对。[结果]获得了不育系21A特异性扩增片段AA/CAG169,该片段中部分序列是Penaeus monodon性别及生长性状相关的AFLP标记序列中的串联重复序列;BLASTX和TBLASTX分析结果表明,该特异性片段的翻译产物与马铃薯的假拟gag-pol蛋白、假拟逆转座子蛋白、假拟逆转座子gag蛋白具有较高的相似性。[结论]特异性片段AA/CAG的功能可能与逆转座子有关,表明其可能与育性密切相关。[Objective] The research aimed to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of pepper by AFLP and lay the foundation for illuminating the molecular mechanism of the cytoplasmic male sterility in pepper further.[Method] Cytoplasmic male sterility （CMS） line 21A and maintainer line 21B were analyzed by using AFLP.The special fragment was reclaimed and sequenced.DNA sequence of the special fragment was analyzed with BLASTn,BLASTX and TBLASTX in GenBank of NCBI.[Result] A special fragment AA/CAG169 was obtained from cytoplasmic male sterility （CMS） line 21A and maintainer line 21B.Partial DNA sequence of AA/CAG169 was the series repeated sequence in AFLP marker sequence related with the sex and growth traits of Penaeus monodon.The results of BLASTX and TBLASTX online showed that AA/CAG169 had higher identities with partial protein sequence of Solanum demissum putative gag-pol protein,Solanum demissum putative retrotransposon protein,and Solanum demissum putative retrotransposon gag protein,respectively.[Conclusion] The function of the fragment AA/CAG169 was closely related to retrotransposon,which indicated that the special fragment could be related to the sterility traits.

关 键 词：辣椒 细胞质雄性不育 AFLP分析 

分 类 号：S641.3[农业科学—蔬菜学]