家蚕神经肽促前胸腺激素基因mRNA的发育表达  

The Developmental Expression of mRNA of Prothoracicotropic Hormone Gene in Bombyx mori

作　　者：于淼[1] 陈复生[2] 魏兆军[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽省农业科学院蚕桑研究所,安徽合肥230061

出　　处：《安徽农业科学》2010年第10期5518-5520,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30500374);教育部新世纪优秀人才基金项目(NCET-07-0251);安徽省优秀人才基金项目(08040106803)

摘　　要：[目的]研究家蚕神经肽促前胸腺激素基因mRNA的发育表达。[方法]采用荧光定量PCR方法,研究家蚕5龄幼虫、预蛹期、蛹期和成虫4个阶段脑—咽下神经节复合体中PTTH基因的mRNA表达量变化。[结果]5龄幼虫PTTH基因mRNA表达量高于预蛹期、蛹期和成虫3个阶段;5龄第6天PTTH基因mRNA表达量最高,蛹期4～8d时持续维持较高表达水平,化蛾前表达量又有所降低,成虫表达量最低。[结论]家蚕PTTH基因mRNA的发育表达变化可能与其调控蜕皮激素合成的功能有关。[Objective]The research aimed to study the development expression of mRNA of prothoracicotropic hormone（PTTH） gene in neuropeptide of Bombyx mori.[Method] By using real-time PCR method,the expression amount changes of mRNA of PTTH gene in the complex of brain and suboesophageal ganglion （Br-SG） in four stages of the 5th larval instar,pre-pupal stage and adult in Bombyx mori were studied.[Result] The expression amount of mRNA of PTTH gene in Br-SGs in the 5th larval instar was higher than that in pre-pupal stage,pupal stage and adult.The mRNA of PTTH gege in the 5th larval instar on the sixth day had the highest expression amount.And it maintained at a higher expression level from the fourth day to the eighth day of pupal stage.And the expression amount before eclosion decreased and reached the minimum value in adult.[Conclusion] The expression changes of mRNA of PTTH gene may realted with the function of regulating the ecdysone synthesis.

关 键 词：家蚕 促前胸腺激素 发育表达 

分 类 号：S881.2[农业科学—特种经济动物饲养]