一种新型可切削生物活性微晶玻璃的生物安全性评价  

Biological safety assessment of a new machinable bioactive glass-ceramic

作　　者：郝玉全[1] 高莹娇[2] 秦小梅[3] 韩雪松[4] 刘敏达[5] 艾红军[1] 

机构地区：[1]中国医科大学口腔医学院口腔修复科,辽宁省沈阳市110002 [2]中国医科大学口腔医学院口腔正畸科,辽宁省沈阳市110002 [3]东北大学材料与冶金学院,辽宁省沈阳市110004 [4]武警辽宁省总队医院口腔科,辽宁省沈阳市110034 [5]中国医科大学口腔医学院,辽宁省沈阳市110002

出　　处：《中国组织工程研究与临床康复》2010年第12期2137-2140,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:自主设计的可切削生物活性微晶玻璃,在保证抗弯强度的同时,有效降低了熔制温度、制备成本。在临床应用前,除必要的机械、理化性能研究外,还需进行生物相容性评估。目的:观察新型可切削生物活性微晶玻璃对小鼠有无全身毒性作用,评价其生物安全性。方法:将纯系昆明种小鼠随机分为实验组、阴性对照组和阳性对照组,实验组经口灌注可切削生物活性微晶玻璃的阿拉伯胶混悬液,阴性对照组灌注等体积阿拉伯胶,阳性对照组灌注醋酸铅溶液,于灌注后24,48,72h观察小鼠一般状态、毒性表现并记录体质量变化。最后处死小鼠取出肝、肾,做光、电镜检查。结果与结论:72h内,实验组小鼠体质量未下降,与阴性对照组比较,差异无显著性意义(P>0.05),肝、肾组织未见病理改变。提示该新型可切削生物活性微晶玻璃经短期口服后对机体无全身毒性。BACKGROUND：The machinable bioactive glass-ceramic we designed recently have a lower melting temperature,and preparation of cost while bending strength in ensuring the same time.Prior to clinical application,biocompatibility should be evaluated.OBJECTIVE：To observe the new machinable bioactive glass-ceramics on the availability of systemic toxicity in mice and evaluate its biological security.METHODS：The Kunming mice were randomly divided into an experimental group,negative control group and positive control group.Mice in experimental group were orally perfused with the suspension of machinable bioactive glass-ceramics and Arabic gum.While the mice in the negative control group were treated with an equal volume of Arabic gum,and the mice in the positive control group were treated with an equal volume of lead acetate solution.The general state and toxicity of mice were observed and body mass was recorded after 24 hours,48 hours,and 72 hours.Finally the mice were sacrificed,and the morphological changes in liver and kidney were then examined by light and electron microscopy.RESULTS AND CONCLUSION：The body mass of mice in the experimental group was not decreased significantly compared with the mice in the negative control group in 72 hours（P 0.05）.Pathological changes of liver and kidney in the experimental group did not be found.The new machinable bioactive glass-ceramic did not show systemic toxicity for a short term.

关 键 词：微晶玻璃 生物安全性 急性全身毒性实验 小鼠 生物材料 

分 类 号：R318[医药卫生—生物医学工程]