镍钛合金表面氧化处理对L-929细胞凋亡相关基因bax/bcl-2 mRNA表达的影响  

Effect of nickel-titanium alloy surface oxidation on L-929-related apoptotic gene bax/bcl-2 mRNA expression

作　　者：冯翠娟[1] 王强[2] 张雪[1] 张扬[1] 秦科[1] 卢利[3] 

机构地区：[1]中国医科大学口腔医学院正畸科,辽宁省沈阳市110002 [2]中国医科大学口腔医学院口腔材料教研室,辽宁省沈阳市110002 [3]中国医科大学口腔医学院口腔颌面外科,辽宁省沈阳市110002

出　　处：《中国组织工程研究与临床康复》2010年第12期2145-2148,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：辽宁省自然科学基金资助项目资助:仿生纳米TiO2覆膜正畸镍钛机械及生物相容性研究(20072106);辽宁省自然科学基金资助项目资助:镍钛合金表面改性及其在口腔正畸学领域的应用基础研究(20082088)~~

摘　　要：背景:镍钛形状记忆合金用于人体最大的问题是合金受到腐蚀后释放出有毒的Ni2+离子,采用各种不同的方法在合金表面制备TiN、TiO2、HA等保护层,可有效抑制Ni离子的溶出,改善其生物相容性。目的:在镍钛形状记忆合金表面制备氧化涂层观察对其生物相容性的影响。方法:将NiTi-SMA板材合金表面进行氧化处理。用10%HF+40%HNO3+50%H2O进行酸洗,丙酮中超声波清洗,干燥,在H2O2溶液中氧化,再经清洗、干燥、灭菌、浸提等处理。L-929细胞体外培养,实验分为阴性对照组:培养液培养;表面氧化组:面氧化处理NiTi-SMA浸提液培养;未处理组:未经处理NiTi-SMA浸提液培养。采用实时反转录聚合酶链反应法检测细胞凋亡相关基因bax/bcl-2 mRNA表达的变化。结果与结论:Bax及bcl-2 mRNA表达阴性对照组均高于表面氧化组和未处理组,而bax/bcl-2比值阴性对照组低于表面氧化组和未处理组,上述结果均具有显著性意义(P<0.01),表面氧化组和未处理组之间bax及bcl-2 mRNA表达及bax/bcl-2比值均无显著性意义(P>0.05)。结果提示,镍钛合金浸提液由于镍离子的释放,可引起L-929细胞的bax及bcl-2 mRNA表达改变,bax及bcl-2 mRNA表达的检测可能成为评价生物材料生物相容性的重要指标。BACKGROUND：Nickel-titanium memory alloy released toxic Ni2＋ following corrosion,which could seriously influence human body.Inhibitory coating of TiN,TiO2,and HA was made at alloy surface,respectively,while the coating might effectively inhibit release of Ni2＋ and improve the biocompatibility.OBJECTIVE：To evaluate the effect of nickel-titanium memory alloy following surface oxidation on biocompatibility.METHODS：NiTi-SMA was treated with surface oxidation.The sample was then rinsed with 10% HF ＋ 40 % HNO3 ＋ 50% H2O,ultrasonic-cleared in acetone,dried,oxygenized in H2O2,rinsed,dried,sterilized,and lixiviated.L-929 cells were cultured in vitro and assigned into negative control（culture medium）,surface oxidation（surface oxidation ＋ NiTi-SMA leaching liquor）,and non-treatment groups（NiTi-SMA leaching liquor alone）.Apoptotic gene bax/bcl-2 mRNA expressions were measured by PT-PCR.RESULTS AND CONCLUSION：bax/bcl-2 mRNA expression in the negative control group was significantly higher than surface oxidation and non-treatment groups,but bax/bcl-2 in the negative control group was significantly lower than surface oxidation and non-treatment groups（P 0.01）.There was no significant difference in bax/bcl-2 mRNA and bax/bcl-2 between surface oxidation and non-treatment groups（P 0.05）.This suggested that the nickel ion releasing from nickel-titanium memory alloy leaching liquor could result in the variation of bax/bcl-2 mRNA expression in L-929,which may be used as an important index to evaluate the biocompatibility of biomaterials.

关 键 词：镍钛形状记忆合金 表面氧化 细胞凋亡 生物相容性 医用智能高分子材料 

分 类 号：R318[医药卫生—生物医学工程]