类风湿关节炎患者外周血高迁移率族蛋白1RORγ／t和白细胞介素-17表达水平的检测及临床意义  

The detection and significance of high mobility group box chromosomal, protein 1, RORγt and inter- leukin-17 in peripheral blood of rheumatoid arthritis

作　　者：石燕[1] 王胜军[1] 陈建国[1] 薛渊[1] 何志强[1] 周成林[1] 郑东[1] 杨恒[1] 李雅贞[1] 仝佳[1] 苏兆亮[1] 邵启祥[1] 许化溪[1] 

机构地区：[1]江苏大学检验医学研究所免疫研究室,镇江212013

出　　处：《中华风湿病学杂志》2010年第3期147-150,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（30871193、30972748）;江苏省卫生厅面上项目（H200859）;江苏大学大学生科研立项（08A017）

摘　　要：目的检测类风湿关节炎（RA）患者外周血高迁移率族蛋白1（HMGB1）及Th17细胞特异性转录因子、相关细胞因子的表达水平，同时分析其与C反应蛋白（CRP）、红细胞沉降率（ESR）和类风湿因子（RF）的关系，初步探讨RA患者HMGB1的表达水平及其与Th17的关系。方法收集80例RA患者外周血标本，其中静止期32例、活动期48例，健康志愿者50名。采用实时荧光定量聚合酶链反应（QRT—PCR）检测外周血单个核细胞（PBMC）中HMGB1、RORγt和白细胞介素（IL）-17的mRNA水平；酶联免疫吸附试验（ELISA）检测血浆HMGB1、IL-17、IL-23的蛋白浓度，采用单因素方差分析和Spearillan相关分析进行统计学处理。结果RA患者PBMC中HMGB1、RORγt和IL一17mRNA的表达水平均高于健康对照组（P〈0．05），且活动期[HMGB1（0．424±0．262）pg／ml，RORγt（0．34±0．25）pg／ml，IL-17（1．42±0．38）pg／ml]明显高于静止期（R0．01）。血浆HMGB1、IL-23和IL-17蛋白水平也显示类似的结果，RA组明显高于健康对照组（P〈0．05），且与CRP、ESR、RF呈正相关（P〈0．05）。结论检测RA患者外周血HMGB1和Th17细胞特异性转录因子及相关细胞因子水平，有助于探讨HMGB1和IL-17在RA发病过程中的病理生理作用，也为寻找RA的治疗靶点提供了新的思路。Objective To detect the expression levels of high mobility group box chromosomal protein 1 （HMGB1） and Th17 cells transcription factors, related cytokines in peripheral blood of rheumatoid arthritis （RA） patients and analyze the relations between HMGB1 and CRP, ESR, RF in RA patients. The other aim of this study is to identify the expression level of HMGB1 and the relationship between HMGB1 and Th17 in RA patients. Methods The mRNA levels of HMGB1, RORγt, interleukin （IL）-17 in the peripheral blood mononuclear cells （PBMC） were determined by quantitative real-time PCR （QRT-PCR） from 80 patients with rheumatoid arthritis,including 32 RA patients in stable phase and 48 patients in active phase, and 50 healthy volunteers. The concentration of HMGB1, IL-23, IL-17 in plasma were detected by enzyme linked immunosorbent assay （ELISA）, one-way ANOVA and Spearman＇s correleation were adopted for statistical analysis. Results The mRNAs of HMGB1, RORγt and IL-17 in RA patients were higher than that in healthy control group （P〈0.05）, especially in active RA patients [ HMGB 1 （0.424±0.262） pg/ml, RORγt （0.34±0.25） pg/ml, IL-17 （1.42±0.35） pg/ml,P〈0.01 ] when compared with patients with stable disease. The concentration of HMGBI, IL-23 and IL-17 in the plasma of RA patients was higher than that of the healthy control group （P〈 0.05）, and was positively correlated with the expression levels of HMGBI, Th17-associated factors and the level of CRP, ESR, RF in RA patients＇ plasma（P〈0.05）. Conclusion The HMGB1 and Th17 cells levels are higher in active RA patients than those in patients with stable disease, arid there is significant positive correlation between them. Detection of peripheral HMGB1 and Th17 cell-specific transcription factors or related cytokines can help to understand the development and progress of rheumatoid arthritis and provide clues for new treatment targets for RA.

关 键 词：关节炎 类风湿 高迁移率族蛋白类 白细胞介素17 

分 类 号：R593.22[医药卫生—内科学]