沙利度胺对系统性红斑狼疮患者外周血T淋巴细胞的免疫调节作用  

The immunoregulatory effect of thalidomide on the peripheral blood T-lymphocytes in systemic lupus erythematosus patients

作　　者：裘影影[1] 李晶[1] 尹筱莹[1] 严利文[1] 殷玉俊[1] 宋晓丽[1] 尤海燕[2] 吴玲[1] 

机构地区：[1]江苏大学附属医院风湿科,镇江212001 [2]江苏大学附属医院中心实验室,镇江212001

出　　处：《中华风湿病学杂志》2010年第3期157-160,共4页Chinese Journal of Rheumatology

基　　金：江苏省科技厅社会发展项目（BS2005043）;镇江市科技局社会发展项目（SH2004040）;镇江市重点医学人才资助项目（2005009）

摘　　要：目的探讨沙利度胺（Thd）对系统性红斑狼疮（SLE）患者外周血T淋巴细胞的免疫调节作用。方法用四甲基偶氮唑蓝（MTT）比色法检测Thd对SLE患者外周血T淋巴细胞增殖的影响，用流式细胞术检测T淋巴细胞早期凋亡及CD3＋CD28＋和CD8＋CD152＋的表达，用实时定量聚合酶链反应（PCR）检测T淋巴细胞白细胞介素（IL）-6、IL-10、肿瘤坏死因子（TNF）-α mRNA的表达，采用单因素方差分析进行统计学处理。结果在体外，500ug／ml Thd组CD3＋CD28＋表达为（48＋9）％显著低于对照组（57±9）％（P〈0．05）、500p．g／mlThd组T淋巴细胞凋亡率为（36＋8）％显著高于对照组（23±5）％（P〈0．05）；100、300、500拉g／mlThd组A570nm值分别为：0．39±0．05、0．34＋0．04、0．30±0．03较对照组（0．51±0．07）均有明显下降（P〈0．05）；100、500ug／mlThd组CD8＋CD152＋表达分别为（5．0±0．6）％、（7．8±0．7）％，明显高于对照组（4．2±0．6）％（P〈0．05）；500ug／ml Thd能抑制IL-6mRNA的表达，各剂量组均抑制IL-10、TNF-α mRNA表达。结论Thd可能通过抑制T淋巴细胞增殖、CD28的表达、IL-6、IL-10、TNF-α mRNA的表达和促进T淋巴细胞凋亡、CD152表达来下调SLE患者的免疫反应。Objective To investigate the immunoregulatory effect of thalidomide on the peripheral blood T-lymphocytes in systemic lupus erythematosus patients in vitro. Methods T-lymphocytes were treated by thalidomide with different concentrations, then the proliferation of these T-lymphocytes proliferation was deteted by MTE while apoptosis and lymphocyte activation marker were analyzed by flow eytometry. The mRNA expression of IL-6, IL-10 and TNF-a was measured by real-time RT-PCR, One-way ANOVA was used for statistical analysis. Results In vitro, thalidomide inhibited the expression of CD3＋CD28＋[500 ug/ml group vs the control group, （48±9）% vs （57±9）% P〈0.05 ]. The pro-portion of apoptotic T-lymphoeytes in the 500 ug/ml group was （36＋8）%, which was significantly higher than that in the control group [ （23±5）%, P〈0.05 ]. The values of A 570nm T-lymphocytes were significantly lower in the 100 ug/ml group, 300 ug/ml group and 500 ug/ml group compared with those of the control group （ 100 ug/ml group vs 300 ug/ml group vs 500ug/ml group vs the control group, 0.39±0.05 vs 0.34±0.04 vs 0.30±0.03 vs 0.51 ±0.07, P〈0.05）, while thalidomide promoted the expression of CD8＋CD152＋ [ 100 ug/ml group vs 500 ug/ml group vs the control group, （5.0±0.6）% vs （7.8±0.7）% vs （4.2±0.6）%, P〈0.05 ]. 500 ug/ml thalidomide inhibited the mRNA expression of IL-6, 2.5-500ug/ml thalidomide inhibited IL-10, TNF-α mRNA expression of T-lymphoeytes. Conclusion Thalidomide can inhibit the proliferative activities and CD28 expression of T-lymphoeytes, reduce mRNA expression of IL-6, IL-10, TNF-α, stimulate CD28 expression and apoptosis of T-lymphocytes. These effects may play an important role in it＇s immune-suppressive effects on systemic lupus erythematosus.

关 键 词：红斑狼疮 系统性 T淋巴细胞 神经免疫调节 沙利度胺 

分 类 号：R593.241[医药卫生—内科学]