系统性红斑狼疮患者CD4＋CD25＋CD127^low/-调节性T细胞中Foxp3的表达及免疫抑制功能研究  

Foxp3 expression in CD4＋CD25＋CD127^low/- regulatory T cells and its immunosuppressive effect in patients with systemic lupus erythematosus

作　　者：宋维亚[1] 李向培[1] 厉小梅[1] 赵书山 马艳[1] 曾小峰[3] 郑颂国[4] 

机构地区：[1]安徽医科大学附属省立医院风湿免疫科,合肥230001 [2]浙江省东阳市人民医院风湿免疫科 [3]中国医学科学院北京协和医院风湿免疫科 [4]美国南加州大学(USC)Keck医学院免疫风湿科

出　　处：《中华风湿病学杂志》2010年第3期168-172,共5页Chinese Journal of Rheumatology

基　　金：国家“十一五”科技支撑计划项目（2008BA159802）;安徽省国际科技合作项目（07080703022）;安徽省临床医学重点学科应用技术项目（05A008）.

摘　　要：目的研究系统性红斑狼疮（SLE）外周血中调节性T细胞不同标志以及调节性T细胞在SLE发病中的作用；探讨CD127与Foxp3的相关性，明确CD127定义调节性T细胞的特异性；鉴定CD4＋CD25＋CD127＋-T淋巴细胞免疫抑制功能。方法①采用四色直接荧光素标记法和多参数流式细胞术检．测40例SLE患者（19例初发和2l例缓解）及15名健康对照外周血CD4＋CD25＋T淋巴细胞、CD4＋CD25＋CD127^low/- T淋巴细胞、CD4＋CD25＋Foxp3＋T淋巴细胞、CD4＋CD25highT淋巴细胞、CD4＋CD25“吒D127^low/- T淋巴细胞、CD4＋CD25^hig Foxp3＋T淋巴细胞以及CD4＋CD127^low/-／-Foxp3＋T淋巴细胞占CD4＋T淋巴细胞的比率，并且将7种调节性T细胞比率与外周血抗双链DNA（dsDNA）等抗体及SLE疾病活动指数（SLEDAI）评分等进行相关性分析。②以流式细胞分选术结合细胞培养技术，检测和分析3例SLE患者和4名健康人外周血中CIM＋CD25＋CD127^low/-调节性T细胞对CD4＋CD25-效应性T细胞增殖的抑制作用。采用两样本均数的t检验，重复测量的方差分析，Pearson相关与Spearman相关分析进行统计学处理。结果①SLE患者组7种调节性T细胞比率分别为（6．1±1．7）％，（3．14±1.3）％，（2．1±1．0）％，（1．6±0．3）％，（0．97±0．28）％，（0．69±0．23）％和（0．71±0．35）％。与健康对照组比较：SLE患者组前6种调节性T细胞比率均低于健康对照组（P〈0．05）。②SLE患者组：CD4＋CD25Toxp3＋、CD4＋CD25＋Foxp3＋T淋巴细胞比率与IgA呈正相关；CD4＋CD25＋CD127^low/-淋巴细胞比率与抗SSB抗体呈正相关。③SLE患者初发组和缓解组比较：SLE患者初发组7种调节性T细胞中除CD4＋CD12T^low/- Foxp3＋T淋巴细胞比率外，其余均低于缓解组（P〈0．05o④SLE患者初发组治疗前后比较：激素治疗前6种调节性T细胞比率均低于激素治疗后（P〈0．05）。⑤SLE患者初发组、缓解�Objective To study the role of peripheral blood T regulatory cells （Treg） markers, as well as different Treg cells in the pathogenesis of systemic lupus erythematosus （SLE） and explore the correlation betweenCD127 and Foxp3. The immunosuppressive effect of-CD4＋CD25＋CD127^low/- T cell is explored. Methods （1） Four-color direct fluorescence-labeled and multi-parameter flow cytometry were used to detect peripheral blood CD4＋CD25＋ T cells and other Treg cells accounted for the proportion of CD4＋ T cells in 40 SLE patients （19 cases with active disease 21 cases in remission） and 15 healthy controls. Meanwhile, its correlations with anti-dsDNA antibodies among 7 groups were analyzed. （1） Flow cytometry sorting combined with cell culture were applied to detect and analyze the proliferation inhibition effect of CD4＋CD25＋CD127^low/-regulatory T cells to CD4 ＋CD25- effector T cell. Two indenpendent samples t test, ANOVA for repeated measures, Pearson＇s correlation and Spearman＇s correlation were used for statistical analysis. Results （2） The cell ratio of the 7 groups of SLE patients was （6.1-＋1.7）%, （3.1-＋1.3）%, （2.1-＋1.0）%, （1.6-＋0.3）%, （0.97-＋ 0.28 ）%, （0.69-＋0.23）% and （0.71-＋0.35 ）% respectively. In the SLE group, the proportion of the first 6 groups was lower than the control group （P〈0.05）. For CD4＋CD25＋CD127^low/- Foxp3＋, there was no difference in the two groups （P〉0.05）. （2） CD4＋CD25＋Foxp3＋, CD4CD25^higFoxp3＋ T cells and IgA ratio was positively correlated, while CD4＋CD25highCD127^low/- T cells and anti-SSB antibodies was positively correlated in SLE patients. （3） The seven group of cells, in addition to CD4＋CD127low/-Foxp3＋ T cell ratio were lower in early-onset SLE patients than those in patients at remission （P〈0.05）. （4） The cell propor-tion of the first 6 groups was lower than that of post steroid treatment （P〈0.05）. （5） Foxp3 expression of CD4＋CD25＋ T cells an

关 键 词：T淋巴细胞 红斑狼疮 系统性 流式细胞术 

分 类 号：R593.241[医药卫生—内科学]