文冠果壳苷对侧脑室注射Aβ_(1-42)致痴呆模型小鼠学习记忆障碍的改善作用  

Protective effects of xanthoceraside on learning and memory impairment induced by intracerebroventricular injections of Aβ_(1-42) in mice

作　　者：迟天燕[1] 王力华[2] 纪雪飞[1] 李伟[1] 汪毅[2] 邹莉波[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第4期314-319,共6页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省教育厅科技项目(20060880);沈阳市科委科技项目(1071060-9-00)

摘　　要：目的考察文冠果壳苷对侧脑室注射β-淀粉样蛋白1-42(amyloid beta peptide fragment 1-42,Aβ1-42)致痴呆小鼠模型学习记忆障碍的改善作用,并初步探讨其作用机制。方法通过Y迷宫和新物体辨别实验检测小鼠的学习记忆能力;使用分光光度计检测脑内丙二醛(malondialdehyde,MDA)含量、谷胱甘肽(glutathione,简称GSH)含量、谷胱甘肽与氧化型谷胱甘肽(glutathione oxidized,简称GSSG)的摩尔比值和总抗氧化能力(total anti-oxidative capacity,T-AOC)的变化。结果与Aβ1-42模型组比较,文冠果壳苷能显著提高Y迷宫实验中小鼠自发交替反应率;显著延长新物体辨别实验中新物体探索时间并提高优先指数。生化结果显示文冠果壳苷能够不同程度地降低Aβ模型小鼠脑内的MDA含量,增加GSH含量,提高GSH与GSSG的摩尔比值,提高T-AOC活性。结论文冠果壳苷对侧脑室注射Aβ1-42小鼠学习记忆障碍具有显著的改善作用,其作用机制可能与对抗自由基损伤,抑制脂质过氧化反应有关。Objective To investigate the effects of xanthoceraside on learning and memory impairment induced in mice by intracerebroventricular（i.c.v.）injection of aggregated peptide β-amyloid 1-42（Aβ1-42）.Methods Learning and memory functions in mice were examined using Y-maze and novel object recognition tests.Biochemical determination of malondialdehyde（MDA）,glutathione（GSH）,the ratio of glutathione and glutathione oxidized（GSH/GSSG）,and T-AOC（total anti-oxidative capacity）were measured with spectrophotometric method.Results Administration of xanthoceraside increased the percentage of alternation behaviors in the Y-maze test and increase the total time of the novel object exploration and preferential index for the novel object in the novel object recognition test in mice impaired by Aβ1-42.Biochemical studies showed that the decrease of tGSH,GSH/GSSG,T-AOC and increase of MDA in mice impaired by Aβ1-42 were significantly ameliorated by xanthoceraside administration.Conclusions The results indicate that xanthoceraside has the effect of improving learning and memory impairment in mice induced by i.c.v.of Aβ1-42 via inhibiting oxidative stress.

关 键 词：文冠果壳苷 阿尔茨海默病 Β-淀粉样蛋白 学习记忆 

分 类 号：R96[医药卫生—药理学]