尾部悬吊大鼠后肢肌肉小动脉内皮血管活性物质及一氧化氮合酶表达的变化  

Vasoactive substance and nitric oxide synthase expression changes of endothelium in arterioles of hindlimb muscle from tail suspended/recovered rats

作　　者：毛秦雯[1] 张立藩[1] 马进[1] 

机构地区：[1]第四军医大学空医系航空生理学教研室

出　　处：《中华航空航天医学杂志》1998年第3期165-168,共4页Chinese Journal of Aerospace Medicine

基　　金：国家自然科学基金

摘　　要：目的阐明模拟失重下血管内皮的变化。方法以尾部悬吊方法模拟失重影响，用免疫组织化学法观察对照（ＣＯＮ）、尾部悬吊４周（ＳＵＳ－４）和恢复１周（ＲＥＣ－１）大鼠后肢肌肉小动脉内皮血管活性物质及一氧化氮合酶（ＮＯＳ）活性的改变。结果在腓肠肌（抗重力肌），模拟失重４周后，ＮＯＳⅡ、ＮＯＳⅢ、Ｐ物质（ＳＰ）及内皮素－１（ＥＴ－１）免疫反应（Ｉｍｍｕｎｏｒｅａｃｔｉｖｅ，ＩＲ）阳性的小动脉长度均较对照组显著减小。其中，又以ＥＴ－１－ＩＲ阳性小动脉长度的相对变化为最大，减少了６０％（Ｐ＜０．０１），ＳＰ－ＩＲ阳性者减少了２７％（Ｐ＜０．０１），ＮＯＳⅡ－ＩＲ与ＮＯＳⅢ－ＩＲ阳性者分别减少了１１％（Ｐ＜０．０５）与４６％（Ｐ＜０．０１）。解除模拟失重１周后，则呈现相反改变，ＳＰ－ＩＲ、ＥＴ－１－ＩＲ阳性者较对照组分别增加了７７％及１７％（Ｐ＜０．０１），而ＮＯＳ则以Ⅱ型－ＩＲ的阳性者变化较大，增加３３％（Ｐ＜０．０１），Ⅲ型－ＩＲ阳性者增加了２８％（Ｐ＜０．０１）。在跖肌（非抗重力肌），各组免疫反应阳性小动脉的长度普遍低于腓肠肌；模拟失重４周后，ＳＰ、ＥＴ－１免疫反应阳性小动脉长度较对照组显著减小，亦以ＥＴ－１－ＩＲ阳性者?Objective To elucidate the effect of simulated weightlessness on endothelial expression of vasoactive substances and nitric oxide synthase. Methods Endothelin 1(ET 1),substance P(SP) and nitric oxide synthase expressions of the endothelum in arterioles of hindlimb muscle from control(CON), 4 wk tail suspended(SUS 4),and 1 wk recovered(REC 1) rats were examined by immunohistochemistry technique. Results Our results showed that the total length of arterioles containing nitric oxide synthase(NOS) type Ⅱ and type Ⅲ,ET 1,and SP in gastrocnemius muscle of SUS 4 group were decreased by 11% ( P <0.05),46%,60%,and 27%( P <0.01) respectively,as compared with that of the CON;however,in REC 1,the total length of them were 33%,28%,17% and 77%( P <0.01) longer than those of the CON respectively. In plantaris muscle,the total length of microvessels containing ET 1 and SP were decreased in SUS 4,and were increased in REC 1 as compared with that of the CON. Conclusion The results suggest that the vasoactive substance and NOS expression of endothelium in hindlimb muscles are lowered during simulated weightlessness and heightened during recovery.These changes are suggested to be implicated in one of the important mechanisms accounting for postflight orthostatic intolerance.

关 键 词：失重模拟 大鼠 一氧化氮合酶 P物质 内皮素-1 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]