应用苯妥英钠后急性低压缺氧条件下大鼠脑单胺递质的变化  

Study on the contents of monoamine neurotransmitters in brain tissue of rats exposed to hypoxia with or without administration of diphenylhydantoin

作　　者：梅丹[1] 董辉[1] 黄晶波[1] 罗卫国[1] 罗军[1] 孙奎[1] 

机构地区：[1]空军医学高等专科学校

出　　处：《中华航空航天医学杂志》1998年第3期169-171,共3页Chinese Journal of Aerospace Medicine

摘　　要：目的探讨苯妥英钠（Ｄｉｐｈｅｎｙｌｈｙｄａｎｔｏｉｎ，ＤＰＨ）在急性缺氧条件下的脑保护作用。方法给大鼠应用ＤＰＨ，用荧光分光法检测其在低压舱模拟升空７．５ｋｍ后１ｈ、２ｈ的脑匀浆单胺递质含量，同时观察脑细胞形态学改变。结果ＤＰＨ实验组在缺氧１ｈ及２ｈ时脑多巴胺和５－羟色胺含量升高（Ｐ＜０．０５），而脑去甲肾上腺素在缺氧１ｈ时降低（Ｐ＜０．０５），缺氧２ｈ恢复正常。电镜形态学观察，低压缺氧条件下对照组大鼠脑细胞水肿，线粒体嵴严重缺失，细胞核明显变形；应用ＤＰＨ的实验组细胞形态基本正常。结论给大鼠应用苯妥英钠后，在急性缺氧条件下，动物脑单胺递质含量的变化可能与缺氧适应或耐受的形成有关。Objective The aim of this study was to investigate the role of diphenylhydantoin(DPH) in protection of brain against acute hypoxia. Methods Seventy two Wistar rats served as subjects. They were divided into 6 groups:3 experimental and 3 control(a. stayed at the ground level,b. underwent 7 500 m hypoxia for 1 h and c.for 2 h).To the experimentals diphenylhydantoin (40 mg/kg) was given ip before hypoxic exposure.The contents of dopamine(DA), noradrenaline(NA) and 5 hydroxytrytamine(5 HT) in the brain tissue were measured by fluoresent method.The histomorphological changes of rat brain were examined by electronmicroscope. Results In experiment als underwent hypoxia for 1 h and 2 h the DA and 5 HT contents of brain tissue were significantly higher than those stayed at ground level.NE content of the rats,underwent 1 h hypoxia was significantly lowered. The differences of DA,NA and 5 HT levels among 3 control groups were nonsignificant.The histomorphological changes of experimental groups were nonsignificant,while swelling of brain cells disappearing of mitochondrial granuals and deformation of nucleus were observed in the control groups underwent hypoxia for 1 h and 2 h. Conclusion It is suggested that DPH is of value in protection of brain against hypoxia,and DA,NA and 5 HT may be involved in the development of tolerance of brain cells to acute hypoxia.

关 键 词：苯妥英 低压 缺氧 脑 去甲肾上腺素 多巴胺 

分 类 号：R852.11[医药卫生—航空、航天与航海医学]