西瓜、甜瓜、黄瓜枯萎病菌遗传多样性UP-PCR分析  

Genetic Diversity Analysis of Fusarium oxysporum Isolated from Watermelon,Melon and Cucumber with UP-PCR

作　　者：张硕[1] 高增贵[1] 庄敬华[1] 赵柏霞[1] 张小飞[1] 那明慧[1] 

机构地区：[1]沈阳农业大学植物保护学院,农业部北方农作物病害免疫重点开放实验室,辽宁沈阳110866

出　　处：《中国蔬菜》2010年第6期19-23,共5页China Vegetables

基　　金：辽宁省科技攻关计划项目(2006215004);辽宁省自然科学基金项目(20062018)

摘　　要：采用Universally Primed PCR(UP-PCR)技术对分离自辽宁省不同地区的西瓜、甜瓜、黄瓜枯萎病株的21株尖孢镰孢菌菌株进行遗传多样性分析。结果表明:6条通用引物共扩增出61条带,其中多态性条带58条,占总条带数的95.1%。通过聚类分析,在相似系数0.746处将21株菌株分为6个类群:FOG1类群包含6株甜瓜尖孢镰孢菌菌株,FOG2包含6株西瓜专化型尖孢镰孢菌菌株,FOG3包含6株黄瓜专化型尖孢镰孢菌菌株,FOG4包含1株西瓜专化型尖孢镰孢菌菌株,FOG5包含1株甜瓜尖孢镰孢菌菌株,FOG6包含1株黄瓜专化型尖孢镰孢菌菌株。Twenty-one isolates of Fusarium oxysporum from watermelon（Citrullus lanatus）,melon（Cucumis melo L.）and cucumber（Cucumis sativus L.）in various areas of Liaoning province were analyzed by UP-PCR.The results indicated that a total of 61 bands were amplified using 6 primers,of which 58 amplified fragments（95.1 %）were polymorphic.The isolates were clustered into six distinct groups at the similarity of 0.746 by cluster analysis.FOG1 group includes six strains of melon,FOG2 group includes six strains of watermelon,FOG3 group includes six strains of cucumber,FOG4 includes a strain of watermelon,FOG5 includes a strain of melon,FOG6 contains a strain of cucumber.

关 键 词：西瓜 甜瓜 黄瓜 枯萎病菌 UP-PCR 聚类分析 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治] S436.421[农业科学—植物保护]