高氟低碘对老年大鼠肺脏细胞DNA损伤的影响  

High-fluoride and Low-iodine Induce DNA Damage in the Lung Cells of Old Rats

作　　者：宁红梅[1] 葛亚明[1] 梁海燕[2] 银梅[1] 

机构地区：[1]河南科技学院动物科学学院,新乡453003 [2]河南新乡医学院,新乡453003

出　　处：《中国畜牧兽医》2010年第4期37-40,共4页China Animal Husbandry & Veterinary Medicine

基　　金：河南省教育厅自然科学项目(2010B230003);河南科技学院博士启动基金(06007);河南科技学院骨干教师基金(200711)

摘　　要：研究高氟低碘对老年大鼠肺脏细胞DNA损伤的影响。健康离乳Wistar大鼠21只,随机分为4组:对照组(5只),饲喂大鼠标准日粮,饮自来水;高氟组(5只),饲喂大鼠对照日粮,饮100 mg/L氟化钠(NaF)去离子水;低碘组(5只),饲喂低碘日粮(定做),饮去离子水;高氟低碘组(6只),饲喂低碘日粮,饮100 mg/L氟化钠(NaF)去离子水。在大鼠20月龄时,处死采取肺脏组织,检查其DNA的损伤情况。高氟组、低碘组和高氟低碘组的老年大鼠肺细胞拖尾率比对照组显著增高(P<0.01),高氟低碘组大鼠肺细胞拖尾率比高氟组、低碘组高,并且均有差异显著性(分别为P<0.01和P<0.05);高氟组、低碘组和高氟低碘组老年大鼠肺细胞的拖尾长度比对照组显著增长,显著性差异分别为P<0.01、P<0.05和P<0.01。高氟低碘组大鼠肺细胞的拖尾长度比高氟组及低碘组增长,与低碘组有差异显著性(P<0.01),但与高氟组差异不显著(P>0.05);高氟组和高氟低碘组老年大鼠肺细胞DNA损伤主要是Ⅲ级损伤(Ⅲ级分别为70.00%和79.31%)。低碘组的肺细胞DNA损伤进入Ⅱ级和Ⅲ级范围的比较多,并且Ⅲ级占有率较高(Ⅱ级为31.25%;Ⅲ级为40.62%)。结果表明,高氟、低碘、高氟低碘都影响了大鼠肺脏细胞的DNA损伤。To study the effects of high-fluoirde and low-iodine DNA damage in the lung of old rats.The experimental models were set up by treating Wistar albino rats with water fluoride（100 mg/L NaF）,diet from the area of low iodine（0.0855 mg/kg）,or both water fluoride and low iodine respectively.The samples of lung were obtained by rats＇ decapitation at 20 months old and were made into unicellular liquid respectively for SCGE.In comparison with DNA damage in the lung cells of the control group 1（58.46%±12.21%）,such DNA damage in the HiF,LI,and HiF＋LI groups 2,3 and 4,was 75.55%±7.69%,87.55%±12.89% and 99.37%±1.76%,respectively.Moreover,the proportion of grade III lung cells damage increased by 70.00% in group 2,40.62% in group 3,and 79.31% in group 4,as compared to the control group 1.These findings indicated that excessive long-term intake of fluoride,with or without adequate I intake,was a significant risk factor for the development of lung damage.

关 键 词：高氟低碘 老年大鼠 肺脏 DNA损伤 

分 类 号：S859.8[农业科学—临床兽医学]