荧光定量PCR方法的原理及其在研究病毒在动物体内分布规律的应用  

The Principle and Application of Fluorescent Quantitation PCR to Research the Distribution Regulation of Virus in Animal Body

作　　者：高均伟[1] 郭延锋[1] 王三虎[1,2] 潘耀谦[1,2] 王丽荣[1,2] 朱国坡[1] 刘兴友[1,2] 

机构地区：[1]河南科技学院动物科技学院,新乡453003 [2]动物疫病和残留物防控河南省高校工程技术研究中心,新乡453003

出　　处：《中国畜牧兽医》2010年第4期63-65,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30771601;30972202);河南省杰出人才创新基金(0521001800);河南省高校科技创新人才支持计划(2009HASTIT010)

摘　　要：研究病毒在动物体内的分布情况,主要是为了掌握病毒在动物体内的生长繁殖规律,找到病毒的靶细胞和靶器官,为进一步研究其致病机理和防制技术提供试验依据。荧光定量PCR法具有定量准确、特异性强、自动化程度高等特点,是研究病毒分布规律的主要方法之一,作者综述了此方法的原理及应用。Researching the distribution of virus in the animal body is mainly to understand the regulation of virus growth and multiplication in animal and find the target cell and target organs of virus.Then it can provide the experimental evidence for the mechanism of disease and control technologies.The method of fluorescent quantitative PCR has the characteristic including quantitative accuracy,specificity,and high degree of automation,etc.So it is one of the main ways to research the distribution.The author summarized the principle and application of this way.

关 键 词：荧光定量PCR 病毒 分布规律 原理 应用 

分 类 号：S854.43[农业科学—临床兽医学]