绵羊Cdkn1c的DNA甲基化分析  

DNA Methylation Pattern of Sheep Cyclin-dependent Kinase Inhibitor 1C

作　　者：赵丽霞[1] 赵高平[2] 郭荣启 王丙萍[1] 周欢敏[1,2] 

机构地区：[1]内蒙古农业大学生物工程学院,呼和浩特010018 [2]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [3]内蒙古呼伦贝尔市农业科学研究所,扎兰屯162650

出　　处：《中国畜牧兽医》2010年第4期85-88,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：为了探究绵羊Cdkn1c的甲基化状态,本研究对绵羊及其它3个物种的Cdkn1c mRNA序列进行了生物信息学分析,并通过亚硫酸氢盐测序法(BSP)对新生羔羊肾脏和肺脏Cdkn1c CpG岛上的CpG位点进行了甲基化分析。结果显示,整个编码区富含CG,为CpG岛;绵羊Cdkn1c所有分析位点均非甲基化,暗示该区域并非差异甲基化区域(DMR)。In order to determine the DNA methylation profile of sheep Cdkn1c,its mRNA sequences of sheep,cattle,human and mouse were subjected to bioinformatic analysis in the present study.Then bisulfite sequencing method（BSP） was used to examine the DNA methylation pattern of CpG island in Cdkn1c.The results of bioinformatic analysis exhibited that the coding region of Cdkn1c was a big CpG island in four species.And bisulfite sequencing results showed that all the analyzed CpG sites were completely non-methylated in the tissues of kidney and lung,suggesting that the analyzed region was not differential methylated region（DMR） in sheep.

关 键 词：Cdkn1c BSP DNA甲基化 印记 

分 类 号：S826[农业科学—畜牧学]