实时定量PCR检测蛋壳形成时骨桥蛋白表达变化的研究  

Expression Level of Detecting OPN by Real-time Quantitative PCR in Eggshell Formation

作　　者：姚秀娟[1] 孙杰[1] 姚守秀[1] 米拉[1] 冯欣璐[1] 赵宗胜[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《中国畜牧兽医》2010年第4期113-118,共6页China Animal Husbandry & Veterinary Medicine

基　　金：石河子大学自然科学与技术创新项目(ZRKX200714)

摘　　要：骨桥蛋白(osteopontin,OPN)是一种具有多种功能分泌型钙结合磷酸化糖蛋白。为了从基因表达的角度来解析蛋鸡蛋壳形成的分子机理,本研究以产蛋鸡为试验动物,采用实时定量PCR技术对与蛋壳形成有关的骨桥蛋白基因在子宫中不同时间段(蛋进入子宫前2 h、进入子宫前1 h、进入子宫后5 h、进入子宫后15 h、产蛋后1 h、产蛋后2 h)的表达丰度进行了研究。结果表明,在蛋进入子宫前2 h、蛋进入子宫前1 h、蛋进入子宫后5 h OPN的表达量逐渐升高,之后在蛋进入子宫后15 h、产蛋后1 h、产蛋后2 h又逐渐下降,这一规律性的变化与蛋壳的钙化密切相关。Osteopontin（OPN） is a kind of multi-functional secretory calcium-binding phosphorylated glycoprotein.In order to resolve the molecular mechanism of the formation of eggshell from the perspective of gene expression,egg-laying hens as the experimental animals in this study,using real-time quantitative PCR technique,to study the abundance expression of the osteopontin gene which was related to eggshell formation in the uterus at different time periods（2 h before egg into the uterus,1 h before into the uterus,5 h after into the uterus,15 h after into the uterus,1 h after egg production,2 h after egg production）.The results showed that 2 h before egg into the uterus,1 h before into the uterus,5 h after into the uterus,OPN expression levels increased gradually;15 h after into the uterus,1 h after egg production,2 h after egg production OPN expression levels decreased gradually.The change of regularity was closely related to egg shell calcification.

关 键 词：蛋壳 荧光定量PCR 骨桥蛋白(OPN) 

分 类 号：S831[农业科学—畜牧学]