维生素C对荷斯坦公牛X精子冻后品质的影响  

Effects of VC on the Quality of Frozen-thawed X-Spermatozoa

作　　者：赵宪林[1] 昝林森[1] 胡建宏[1] 田万强[2] 孙永刚[3] 郭红芳[3] 桂林生[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]杨凌职业技术学院,杨凌712100 [3]西北农林科技大学动物医学学院,杨凌712100

出　　处：《中国畜牧兽医》2010年第4期121-124,共4页China Animal Husbandry & Veterinary Medicine

基　　金："十一五"国家科技支撑计划(2006BAD01A10-03);陕西省"13115"科技专项(2007ZDCY-01)

摘　　要：试验旨在研究维生素C(VC)对荷斯坦公牛性控精液冻后品质的的影响。在精液稀释液中分别添加0,2.0,4.0,6.0,8.0 mg/mL的VC进行试验。将装有X精子的细管冻精解冻(37.5℃,30 s)后分析0～3 h精子活率及0～4 h质膜完整率和顶体完整率的变化情况。结果表明,当添加量为6.0 mg/mL时,解冻后0 h精子活率与对照无差异显著,解冻后1、23、h精子活率与对照组差异显著(P<0.05)。当VC的添加量为4.0 mg/mL时,解冻后0 h时X精子质膜完整率显著高于对照组(P<0.05),解冻后1 h与对照相比差异不显著(P>0.05),但数值依然高于对照组。解冻后存放2、34、h与对照组相比差异显著。添加量为6.0 mg/mL时,解冻后0 h时X精子顶体完整率显著高于对照组(P<0.05),解冻后1、3 h精子顶体完整率与对照组相比差异显著(P<0.05),24、h与对照相比差异不显著(P>0.05),但顶体完整率高于对照组。在冷冻精液稀释液中VC的适宜添加量为4～6 mg/mL,此浓度下可有效提高荷斯坦公牛X精子解冻后活率、质膜完整率及顶体完整率。The aims of this study were to determine the effects of VC on protection of frozen-thawed X-sperm.The different concentration of VC（0.0,2.0,4.0,6.0,8.0 mg/mL） were added in ordinary dilution for experimentize.After thawed in thermostat-controlled waterbath about 37.5 ℃ for 30 s,then to detect the changes of sperm motility and the percent of integrity perforatorium and plasma membrane integrity.The results showed that the concentration of VC as 6.0 mg/mL,the sperm motility compare with the control group indicate no differences（P〈0.05） when after frozen for 0 h,and it indicated differences（P〈0.05） compare withd control group when after frozen for 1,2 and 3 h.when the concentration of VC as 4.0 mg/mL have the good effect on the rate of the sperm plasma membrane integrity.It indicate differences（P〈0.05）compare with the control group.When frozen for 0 hour,and indicated no difference compare with che control group（P〈0.05）.When the sperm frozen for 1 h,and frozen for 2,3,4 h,the volue indicate difference compare with the control（P〉0.05）.When the concentration of VC as 4.0 mg/mL had the good effect on the rate of the sperm acrosome integrity.It indicated differences（P〈0.05）compare with the control group.When frozen for 0 h,and indicate no differenced compare with che control group（P〉0.05）.When the sperm frozen for 1,3 h,and frozen for 2,4 h,the volue indicated no difference compare with the control group（P〉0.05）,but the volue was higher than control group.These results indicated that added the sufficient VC as 4 to 6 mg/mL in dilution of frozen semen can rise X-sperm quality effectily after frozen-thawed.

关 键 词：荷斯坦公牛 冷冻精液 维生素C X精子 

分 类 号：S814.8[农业科学—畜牧学]