山羊胚胎体外培养条件的优化  

Optimization of Goat Embryos in vitro Culture Conditions

作　　者：刘羿羿[1] 赵高平[2] 李燕[3] 白俊[2] 王申元[2] 巴特尔[2] 周欢敏[4] 

机构地区：[1]内蒙古农业大学生物工程学院,呼和浩特010018 [2]内蒙古农业大学动物科学学院,呼和浩特010018 [3]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010021 [4]内蒙古农业大学科技处,呼和浩特010018

出　　处：《中国畜牧兽医》2010年第4期146-149,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金(30460091)

摘　　要：为优化山羊胚胎体外培养条件,本试验比较了含有3种不同来源血清(优质胎牛血清、发情山羊血清、前列腺素(PG)处理后发情山羊血清)的M199对卵母细胞成熟效果的影响,成熟率分别为65.95%、49.2%、76.47%,差异极显著(P<0.01);本试验还分别采用了SOFaa、CR1aa+输卵管上皮细胞共培养,以及改进的DMEM/F12发育体系培养受精卵,结果发现,SOFaa、CR1aa+输卵管上皮细胞的发育培养系统得到的囊胚率为29.62%、24.73%,差异不显著(P>0.05),改进的DMEM/F12发育液囊胚率最高为48.42%,差异极显著(P<0.01)。This experiment compared the effect of mature liquid that containing three different sources serum： high-quality fetal bovine serum,estrus goat serum,PG treated estrus goat serum,maturation rate were 65.95%,49.2%,76.47%（P〈0.01）respectively,the difference was extremely significant;compared SOFaa,CR1aa＋ oviduct epithelial cells,and improved DMEM/F12 development system and found that,the blastocyst rate of SOFaa,CR1aa＋ oviduct epithelial cells were 29.62%,24.73%,difference was not significant（P〉0.05）,the DMEM/F12 system had higher blastocyst development rates 48.42%,the difference was very significant（P〈0.01）.

关 键 词：发情山羊血清 囊胚率 输卵管上皮细胞 

分 类 号：S814[农业科学—畜牧学]