苏太猪抗F18^+大肠杆菌病基础群FUT1基因多态性及其与生长发育的关联分析  

Polymorphism of FUT1 Gene and its Relationship with Growth and Development in Foundation Colony of Sutai ETEC F18^+ Resistant Breeding Pigs

作　　者：叶兰[1] 潘章源[1] 黄雪根 华金第 吴圣龙[1,3] 包文斌[1,3] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省苏州市苏太猪育种中心,苏州215000 [3]江苏省扬州规模猪场高效健康养殖公共技术服务中心,扬州225009

出　　处：《中国畜牧兽医》2010年第4期158-161,共4页China Animal Husbandry & Veterinary Medicine

基　　金：转基因生物新品种培育科技重大专项(2009ZX08006-004B);江苏省科技支撑计划(农业)(BE2008364);江苏省高校自然科学基础研究项目(08KJB230004)

摘　　要：本研究采用PCR-RFLP方法对苏太猪F18+大肠杆菌抗病育种基础群α-(1,2)岩藻糖转移酶基因1(FUT1)M307位点多态性进行了分析,并探讨了其与部分生长发育性能的相关性。苏太猪F18+大肠杆菌抗病育种基础群FUT1基因M307位点经Hin6Ⅰ酶切后,产生AA、AG和GG 3种基因型,频率分别为0.235、0.609和0.156,群体极显著的偏离了Har-dy-Weinberg平衡状态(P<0.01)。总体上AA基因型的个体生长发育优于AG和GG基因型的个体,其中AA基因型个体的60 kg体长显著高于AG和GG基因型的个体(P<0.05),断奶重显著高于GG基因型的个体(P<0.05),体高和后躯宽显著高于AG基因型的个体(P<0.05)。试验结果表明,苏太猪抗病育种基础群中FUT1基因第307位点的AA基因型不仅对仔猪断奶后水肿病和腹泻病具有抗性,而且具有较高的早期生长发育性能。The genetic variations at M307 of FUT1 gene in foundation colony of Sutai ETEC F18^＋ resistant breeding pigs （total 576 individuals） were investigated by PCR-RFLP method. The relationship between FUT1 gene and some traits of growth and development were analyzed in this study. The results showed that M307 of FUT1 gene digested by Hin6 I could be divided into three kinds of genotypes（AA, AG and GG）. The genotypic frequencies of FUT1 locus were 0. 235 （AA）, 0. 609 （AG） and 0. 156（GG） respectively, this locus deviates from Hardy-Weinberg equilibrium（P〈0.01） in above foundation colony. The correlation between three genotypes and the performances showed that the growth and development of individuals with AA genotype were higher than those of individuals with AG and GG genotypes. Body length of pigs with genotype AA was significantly higher than that of AG and GG genotypes. The 35-days body weigh of individuals with AA genotype was significantly higher than those with GG genotype. Body height and rear quarters width of AA genotype were significantly higher than those with AG genotype. The results indicated that AA genotype at M307 of FUT1 gene had good genetic effects on disease resistance traits of PWD and PED, as well as some early productive performances. And such association could be applied in the ge- netic breeding practice in the near future.

关 键 词：FUT1基因 生长发育 苏太猪 抗病育种 

分 类 号：S813.24[农业科学—畜牧学]