血清同型半胱氨酸水平、亚甲基四氢叶酸还原酶基因多态性与2型糖尿病合并脑梗死关系的研究  

Association of homocysteine,methylenetetrahydrofolate reductase gene polymorphism with type 2 diabetes mellitus with cerebral infarction

作　　者：曹宏[1] 黄德芳[1] 毛莉[2] 高玉芬[1] 

机构地区：[1]无锡市第三人民医院内分泌科,江苏无锡214041 [2]淮安市第一人民医院内分泌科,江苏淮安223300

出　　处：《中华疾病控制杂志》2010年第3期196-198,共3页Chinese Journal of Disease Control & Prevention

摘　　要：目的探讨血清同型半胱氨酸水平、亚甲基四氢叶酸还原酶(MTHFR)基因的分布情况与2型糖尿病合并脑梗死的关系。方法利用聚合酶链反应和限制性片断长度多态性(PCR-RELP)方法检测了41例无并发症的糖尿病患者和42例2型糖尿病合并脑梗死患者MTHFR基因的677碱基多态性突变C→T情况,同时测定其血清同型半胱氨酸(Hcy)的水平,并加以对照分析。结果2型糖尿病合并脑梗死患者血清Hcy水平明显高于无并发脑梗死的糖尿病患者,但MTH-FR基因突变型C677T基因的频率与无并发症的糖尿病组相比差异无统计学意义。在叶酸浓度≤6.92nmol/L时,2型糖尿病合并脑梗死组TT型发生频率及T等位基因频率高于无并发症的糖尿病组(P<0.05)。结论糖尿病患者中MTHFR基因C677T突变者有发生糖尿病并发脑梗死的倾向,但高叶酸浓度可以明显降低这种倾向。Objective To evaluate the role of homocysteine, methylene tetrahydrofolate reductase （MTHFR） gene polymorphisms in type 2 diabetes mellitus with cerebral infarction. Methods By PCR- RFLP, MTHFR C677T mutation was detected in 42 type 2 diabetes mellitus with cerebral infarction and 41 type 2 diabetes mellitus with no complications as control. The serum homocysteine （Hey） levels were measured by enzyme-labeled immunoassay （ELISA）. Results The serum Hey levels of type 2 diabetes mellitus with cerebral infarction was higher than type 2 diabetes mellitus with no complications. There was no significant difference of the frequency of MTHFR C677T gene between the two groups. In subject with serum folate ≤6.92 nmol/L, the frequency genotype TT and allele frequency T of type 2 diabetes mellitus with cerebral infarction was higher than that of control group （ P 〈 0.05）. Conclusions Diabetic patients with MTHFR gene C677T rnutation is predisposed to developing cerebral infarction, and the high-normal serum folate may prevent the progression of cerebral infarction.

关 键 词：亚甲基四氢叶酸还原酶 同型半胱氨酸 糖尿病 2型 脑梗死 

分 类 号：R587.29[医药卫生—内分泌]