微生物源追踪技术在不同污染水体中的应用评价  

The application and evaluation of microbial source tracking method in different polluted water

作　　者：于洋[1] 顾玲[2] 张婷婷[3] 王毓[1] 丁震[2] 叶珣[3] 周璐[2] 汪华[2] 

机构地区：[1]南京医科大学公共卫生学院流行病与卫生统计学系,江苏南京210029 [2]江苏省疾病预防控制中心,江苏南京210009 [3]东南大学公共卫生学院,江苏南京210009

出　　处：《中华疾病控制杂志》2010年第3期260-262,共3页Chinese Journal of Disease Control & Prevention

基　　金：江苏省"333工程"培养资金

摘　　要：目的建立南京秦淮河上游中山河和淮河流域桂五水库的微生物源追踪抗生素敏感性分析(antibiotic resistance analysis,ARA)数据库,追踪水体中的污染来源,探讨该方法在不同水体中追踪微生物污染源的应用价值。方法采集目标水体周边自然村中人、家畜、家禽等的粪便标本及生活和工业污水排放口直接排放的污水,分离并确认指示菌,建立已知源微生物源追踪数据库。按计划采集未知源水样,分离指示菌并使用J MP7.0软件进行污染源追踪及效果评价。结果秦淮河上游中山河水体污染源主要为工业及生活污水和狗的粪便污染。淮河流域桂五水库环境水污染源则主要为人和羊的粪便污染。结论微生物源追踪方法不仅可以应用于原生态水体,而且可以应用于受生活、工业双重污染水体的微生物污染源追踪,均能取得较好的效果。Objective To set up antibiotic resistance analysis database of Nanjing Zhongshan River and Guiwu reservoir of the Huai River, track the source of the pollution in two different water body and evaluate the results of them. Methods Fecal samples, industrial and domestic waste water samples were collected around the objective waterbodys. Indicator bacteria were isolated from these samples, microbial source tracking database were built. Discriminant analysis with JMP7. 0 software were used to track pol- lution sources of the water and evaluate the result. Results The major pollution source of zhongshan River were waste water and fece of dog. The major pollution source of Guiwu reservoir were feces of human and sheep. Conclusions The antibiotic resistance analysis as a microbial source tracking method is available for both ecosystem water body and dual polluted water body.

关 键 词：微生物源追踪 抗药性 微生物 水污染 

分 类 号：R117[医药卫生—公共卫生与预防医学]