2003-2008年河北省H3N2亚型流感病毒HA1基因演变特征  

Evolutionary characterization of HA1 gene of influenza A H3N2 viruses from 2003 to 2008 in Hebei

作　　者：齐顺祥[1] 刘艳芳[1] 韩光跃[1] 刘兰芬[1] 高伟[1] 

机构地区：[1]河北省疾病预防控制中心病毒病防治所,河北石家庄050021

出　　处：《中华疾病控制杂志》2010年第4期313-315,共3页Chinese Journal of Disease Control & Prevention

摘　　要：目的了解近几年河北省H3N2亚型流感病毒HA1基因演变概况。方法选取2003-2008年河北省分离的25株H3N2亚型流感病毒,提取病毒RNA,进行逆转录-聚合酶链反应扩增,产物纯化测序,测定的序列用生物信息软件分析。结果每个流行期分离的毒株都较当年的疫苗株或前一年的毒株出现了位于不同抗原决定簇或者受体结合位点的氨基酸替换。2003-2008年不同流行期分离株在3、140、142、144、145、158、159、189、192、193、198、204、225、226和227位点氨基酸发生了变化,多数的改变发生在抗原决定簇A、B及RBS。进化树分析显示,每个流行期分离株均与前一年的毒株或当年疫苗株出现了不同程度的变化,不断出现新的进化分支,同一流行期分离株基本呈现集中分布。结论2003-2008年河北省H3N2亚型流感病毒HA1基因不断发生变异,密切关注其变异对防控流感流行有重要意义。Objective To understand the genetic characterization of HA1 gene of influenza A H3N2 viruses circulated in recent years in Hebei. Methods Viral RNAs of 25 H3N2 strains were extracted and amplified by Reverse Transcription Polymerase Chain Reaction （RT-PCR）. The products of PCR were purified and sequenced, and the sequences were analyzed though biometic software. Results Sev- eral amino acid substitutions located in antigenic sites or receptor binding sites were found more in the isolates than the current vaccine virus or the isolates from previous year. Amino acid substitution was found in 3, 140, 142, 144, 145, 158, 159, 189, 192, 193, 198,204, 225, 226 and 227 positions in the isolates during 2003 - 2008, more amino acid substitutions took place in antigenic determinant A, B and receptor binding site （RBS）. New phylogenetic branches appeared continuously during 2003- 2008. The H3N2 strains of the same year almost clustered in the same group on the phylogenetic tree. Conclusions Amino acid substitutions continuously occurred in the HA1 genes in influenza A H3N2 viruses isolated in Hebei from 2003 to 2008, it is meaningful to pay close attention to the HA1 variation in order to prevent and control influenza.

关 键 词：流感病毒A型 H3N2亚型 基因 抗原变异 河北 

分 类 号：R373.13[医药卫生—病原生物学] R394.112[医药卫生—基础医学]