小鼠BPI N端功能片段(muBPI_(25)蛋白)体外杀菌和中和内毒素作用研究  

Studies on antibacterial activity and endotoxin neutralization of murine BPI N-terminal functional fragment(muBPI_(25) protein)in vitro

作　　者：吕喆[1] 王炜[1] 范宜强[1] 刘振龙[1] 孔庆利[1] 温铭杰[1] 龙军[1] 李晨[1] 许晴[1] 安云庆[1] 

机构地区：[1]首都医科大学基础医学院免疫学系,北京100069

出　　处：《中国免疫学杂志》2010年第4期294-297,303,共5页Chinese Journal of Immunology

基　　金：北京市自然科学基金项目(7082016)资助

摘　　要：目的:建立胞内杀菌和体外中和内毒素实验模型,检测muBPI25目的蛋白对胞内寄生菌的抑杀作用和对内毒素的中和作用。方法:将pcDNA3.1(+)-muBPI36-259质粒导入RAW264.7细胞,用胞内寄生G+/G-菌感染上述细胞建立muBPI25目的蛋白胞内杀菌实验模型;将pSecTag2B-muBPI36-259与双荧光素酶报告基因质粒共转染RAW264.7细胞,建立体外检测muBPI25蛋白中和内毒素实验模型。结果:胞内杀菌实验模型证实muBPI25目的蛋白对G-伤寒杆菌具有抑杀作用;中和内毒素实验模型证实muBPI25目的蛋白对内毒素具有中和作用。结论:首次证实小鼠BPI N端功能片段,即muBPI25目的蛋白对G-菌具有抑杀作用,对其裂解产物内毒素具有中和作用。Objective：To establish an experimental model for intracellular antibacteria and endotoxin neutralization in vitro to detect the antibacterial and endotoxin neutralization activity of the muBPI25 protein.Methods：RAW264.7 cells were transfected with pcDNA3.1（＋）-muBPI36-259,and then were infected with intracellular bacterial of either G＋/G to establish the experimental model of intracellular antibacteria.The RAW264.7 cells were co-transfected with the pSecTag2B-muBPI36-259 and dual-luciferase reporter gene plasmids for establishment of the experimental model of endotoxin neutralization.Results：The experimental model of intracellular antibacteria confirmed that the muBPI25 protein could inhibit/kill Salmonella typhi.The experimental model of endotoxin neutralization indicated that the muBPI25 protein could neutralize endotoxin.Conclusion：We firstly demonstrate that murine BPI N-terminal functional fragment（muBPI25 protein） can inhibit/kill Salmonella typhi,and can neutralize its lysating product,endotoxin.

关 键 词：杀菌/渗透性增强蛋白 RAW264.7细胞 胞内寄生菌 内毒素 

分 类 号：R392[医药卫生—免疫学]