HLA-DRB1基因多态性与慢性髓性白血病的相关性研究  

Association between HLA-DRB1 allele polymorphism and susceptibility to chronic myelogenous leukemia in Chinese Han

作　　者：霍明瑞[1,2] 刘海燕[1,2] 于燕[1,2] 郗斌[1,2] 黄晓军[1,2] 李丹[1,2] 

机构地区：[1]北京大学人民医院 [2]北京大学血液病研究所HLA配型室,北京100044

出　　处：《中国免疫学杂志》2010年第4期309-311,共3页Chinese Journal of Immunology

基　　金：教育部创新团队发展计划基金(No.IRT0702);国家高科技研究发展项目(863计划)(No.2006AA02A405)

摘　　要：目的:探讨HLA-DRB1等位基因多态性与慢性髓性白血病(CML)关联性。方法:采用序列特异性引物聚合酶链反应(PCR-SSP)DNA分型技术对762例慢性髓性白血病患者(男性492例,女性270例)及2 264例正常对照进行了HLA-DRB1基因分型。结果:正常人群的HLA-DRB1*15等位基因频率最高(17.25%),其次为DRB1*09(14.05%)、DRB1*12(11.73%)和DRB1*04(10.98%),DRB1*10等位基因频率最低(1.39%)。CML患者HLA-DRB1*08等位基因频率显著高于正常对照组(7.48%vs 5.39%,χ2=8.963,OR=1.023,P=0.004),男性患者的DRB1*08基因频率也明显高于正常对照(7.72%vs 5.39%,χ2=8.059,OR=1.025,P=0.007)。女性患者的DRB1*08基因频率也高于正常对照(7.04%vs 5.39%,χ2=0.115,OR=0.995,P=0.774),但没有统计学差异。结论:CML男性患者HLA-DRB1*08的表达明显高于正常人群,提示DRB1*08可能是男性CML患者的易感基因。Objective：The aim was to explore the association between the HLA-DRB1 allele polymorphism and susceptibility to chronic myelogenous leukemia（CML） in Chinese Han population.Methods：The polymorphism of HLA-DRB1 alleles in 762 CML patients and 2 264 normal umbilical cord blood samples were tested by polymerase chain reaction with sequence specific primer（PCR-SSP）.Results：The most common allele in DRB1 locus in normal population was DRB1＊15（17.25%）,whereas DRB1＊10 was the least frequent allele（1.39%）.Compared with normal controls,the frequency of HLA-DRB1＊08 was significantly increased in all CML groups（7.48% vs 5.39%,χ2=8.963,OR=1.023,P=0.004） and CML male groups（7.72% vs 5.39%,χ2=8.059,OR=1.025,P=0.007）.But there was no significant difference between the normal controls and CML female patients.Conclusion：It should be concluded that DRB1＊08 could be considered a susceptible allele for CML male patients.

关 键 词：HLA-DRB1 慢性髓性白血病 基因多态性 基因频率 

分 类 号：R392.2[医药卫生—免疫学] R551.3[医药卫生—基础医学]