CEA抗原表位串联体与FL共表达基因疫苗抑制小鼠肝癌细胞体内生长的研究  

The inhibitory effects of the DNA vaccine co-expressing CEA tandem repeat epitopes and FL on the in vivo growth of heptocarcinomas in mice

作　　者：段秀梅[1] 车媛媛[1] 黄晶[1] 石旭[1] 李丹[1] 刘力华[1] 方艳秋[1] 谭岩[2] 宋燕[2] 

机构地区：[1]吉林大学第一医院中心实验室,长春130021 [2]吉林大学中日联谊医院普通外科,长春130033

出　　处：《中国免疫学杂志》2010年第4期315-319,共5页Chinese Journal of Immunology

基　　金：吉林省科技厅重点资助项目(20050402-3)

摘　　要：目的:观察CEA抗原表位串联体与FL共表达基因疫苗抑制荷瘤小鼠肿瘤生长。方法:利用基因重组技术将CEA抗原表位三串联体和FL基因片段克隆到质粒pcDNA3.0上,肌注免疫BALB/c小鼠,观察重组基因疫苗对CEA阳性肿瘤的抑制作用、小鼠生存时间及其诱导小鼠杀伤效应细胞的活性。结果:pcDNA-triCEA625-667-sFL免疫组小鼠生存时间延长,肿瘤生长速度缓慢,瘤块较小,与对照组小鼠比较有显著性差异(P<0.01);经pcDNA-triCEA625-667-sFL免疫的小鼠脾细胞对H22-CEA+的杀伤率明显升高,差异有显著性(P<0.01)。结论:共表达三倍体CEA抗原表位和FL的基因疫苗可以有效抑制CEA阳性肿瘤在小鼠体内的生长,并增强小鼠杀伤效应细胞的活性。Objective：To observe inhibitory effects of DNA vaccine co-expressing CEA tandem repeat epitopes and FL on cancer cells in mice.Methods：The encoding sequences for CEA tandem repeats and FL were inserted into plasmid pcDNA3.0 using gene recombinant technique.BALB/c mice were immunized intramuscularly with the co-expressing DNA vaccine.The survival time and tumor size were measured and specific CTL cytotoxicity was detected by 125 I-UdR release method.Results：Compared with that of the control,the survival time was prolonged（P0.01）and the tumors were significantly inhibited in the mice immunized with the vaccine pcDNA-triCEA625-667-sFL（P0.01）.The splenic cells from mice immunized with the vaccine pcDNA-triCEA625-667-sFL induced strongly cytotoxicity against tumor cells H22-CEA＋（P0.01）.Conclusion：The recombinant DNA vaccine co-expressing pcDNA-triCEA625-667-sFL can suppress the growth of tumor expressing CEA in mice and enhance CTL response against CEA antigen.

关 键 词：癌胚抗原 FLT3配体 抗原表位 基因疫苗 

分 类 号：R392.11[医药卫生—免疫学]