雌、孕激素对小鼠uNK细胞在子宫壁内分布的影响  

The effects of estrogen and progesterone on distribution of uNK cells in mouse uterus

作　　者：徐宏[1] 余克花[2] 张保平[2] 彭婧[1] 张莉[1] 况海斌[1] 

机构地区：[1]南昌大学医学院生理学教研室,南昌330006 [2]南昌大学医学院实验教学部,南昌330006

出　　处：《中国免疫学杂志》2010年第4期330-334,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(30760071)

摘　　要：目的:探讨甾体激素对小鼠子宫自然杀伤(uterine Natural Killer,uNK)细胞在子宫壁内分布的调节作用。方法:用uNK细胞特异性的DBA-lectin抗体,对去卵巢以及卵巢甾体激素处理的小鼠子宫进行免疫组织化学标记。结果:在去卵巢小鼠给予雌二醇后,uNK细胞主要分布于子宫的基质,为一些小的、圆形细胞;给予孕酮后,DAB-lectin染色主要见于一些血管内皮细胞;联合给予雌二醇和孕酮后,DBA-lectin染色分布于子宫的基质,既可见于一些小的、圆形uNK细胞,也见于一些血管内皮细胞。雌、孕激素作用均可被其相应受体的拮抗剂所阻断。结论:雌、孕激素对小鼠uNK细胞在子宫壁内分布的具有协同性的调节作用,共同参与妊娠子宫对半同种异体胚胎的保护性免疫反应。Objective：To investigate the effect of steroid hormones on distribution of uterine natural killer（uNK） cells in the mouse uterus.Methods：A unique uNK cell marker,Dolichos biflorus agglutinin（DBA） lectin was used to localize uNK cells in the ovariectomized and ovarian steroid hormone-treated mouse uterus by immunohistochemical staining.Results：After estrogen（E2） was administered in the ovariectomized mice,uNK cells were distributed in the stroma of uterine mesometrial pole,as round,immature and small lymphocyte-like cells.With the progesterone（P4） administered,the immunostaining results showed that DAB-lectin staining were mainly distributed in the vascular endothelial cells.With the combination of E2 and P4,DAB-lectin staining was distributed in the matrix of the uterus,seen as a number of small round uNK cells or some as vascular endothelial cells.The effects could be completely abolished by specific antagonists of their nuclear receptors（estrogen and progesterone receptor）.Conclusion：The distribution of uNK cells in mouse uteri is collaboratively regulated by estrogen and progesterone.The endometrial uNK cells may be involved in the mechanism of the fetal protective immune reaction during pregnancy.

关 键 词：UNK细胞 子宫 雌激素 孕激素 

分 类 号：R977.12[医药卫生—药品]