银杏叶化学成分的裂解甲基化气相色谱分析  

Determination of multi-chemical constitutes for Ginkgo biloba leaves by gas chromatography with pyrolytic methylation

作　　者：王丽丽[1] 周彦[1] 贾以律[1] 唐春兰[1] 周汉君[1] 潘再法[1] 

机构地区：[1]浙江工业大学化学工程与材料学院,浙江杭州310032

出　　处：《浙江工业大学学报》2010年第2期124-128,共5页Journal of Zhejiang University of Technology

基　　金：浙江省自然科学基金资助项目(Y307602);浙江省科学技术厅分析测试科技计划项目(2007F70065)

摘　　要：应用纵型微型炉裂解器直接裂解甲基衍生化-气相色谱法同时测定银杏叶中数种化学成分．样品粉末与衍生化试剂TMAH（25％甲醇）同时注入裂解器进样口，在300℃的进样口瞬间生成了包括5种酚酸类化合物、6种脂肪酸和9种烷基酚在内的甲基衍生物．各种成分的RSD为0．5％～5．9％（n=3）．该方法无需任何样品前处理，样品用量少，为银杏叶中多种化学成分同时测定提供一种快速、简便和准确的方法．Pyrolytic methylation-gas chromatography using a vertical microfurnace pyrolyzer was applied to analysis of multi-chemical constitutes for G. biloba leaves simultaneously and directly. Pyrolytic methylation of G. biloba leaves in the injector at 300 ~C in the presence of tetramethylammonium hydroxide （TMAH）, allowed quantitative analysis of 5 kinds of phenols and carboxylic acid, 6 kinds of fatty acid and 9 kinds of alkylphenol as their corresponding methyl derivatives, with the relative standard deviations （RSDs） less than 5.9% （n=3）. The developed method allows the analysis small samples without laborious pre-treatment and would be a simple, accurate and highly sensitive way to detect multi-constitutions in Ginkgo biloba leaves.

关 键 词：气相色谱 在线甲基衍生化 银杏叶 酚酸类 脂肪酸 烷基酚 

分 类 号：O657.71[理学—分析化学]