绿僵菌对甘蔗螟虫的室内致病力测定  

Bioassay of Toxicity of Metarhizium spp.to Sugarcane stemborers in Laboratory

作　　者：刘磊[1,2] 梁昌聪[1] 杨腊英[1] 黄志[1,2] 陆红霞[2] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所海南省热带农业有害生物检测监控重点实验室,海南儋州571737 [2]海南大学农学院,海南儋州571737

出　　处：《热带作物学报》2010年第2期235-240,共6页Chinese Journal of Tropical Crops

基　　金：非营利性科研机构启动费(No.HZS0807);国家科技支撑计划重点项目(No.2007BAD48B02);农业科技成果转化项目(No.2007GB23260410);中央级公益性科研院所基本科研业务费专项(No.2009HZS1J003)资助

摘　　要：在室内用喷雾法测定了4株绿僵菌对甘蔗螟虫3～4龄若虫的致病力。结果表明,绿僵菌致病力强弱与菌株种类和接种剂量有密切关系。先接虫后喷施菌剂时,菌株对甘蔗螟虫若虫致病力的强弱顺序为JF813>HS1>MA4>JF883;先喷施菌剂后接虫时,菌株对甘蔗螟虫若虫致病力的强弱顺序为MA4>HS1>JF813>JF883。在接种各菌株孢子浓度为1×108个/mL时,菌剂对甘蔗螟虫若虫的致死率高。其中JF813菌株对甘蔗螟虫若虫具有很强的二次侵染能力,HS1菌株在两种处理方式中致病力稳定。The third to fourth instar nymphs of sugarcane stem borers were sprayed with solutions of 4 strains of fungus Metarhiziums spp.in laboratory to observe the pathogenicity of each strain against the nymphs.The bioassay showed that the pathogenicity of the fungus was closely related with the strains and their inoculation rate.The pathogenicity of the fungus was in the order of the strains JF813﹥HS1﹥MA4﹥ JF883 when sugarcane was inoculated with the larvae of sugarcane stemborers first and then with the fungus,and MA4﹥HS1﹥JF813﹥JF883 when vice versa.The mortality of sugarcane stem borers was the highest when sugarcane was inoculated with fungus at a concentration of 1×108 spores·ml-1.The strain JF813 had a very high ability in infecting the nymphs the second time,and the HS1 had a stable pathogenicity.

关 键 词：绿僵菌 甘蔗螟虫 致病力测定 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]