DECAY OF BLOOD IN VITRO IRRADIATED BY LASER  

激光照射离体血液的衰变规律(英文)

作　　者：张枫[1] 刘莹[1] 杨成方[1] 李雷[1] 

机构地区：[1]徐州师范大学物理与电子工程学院

出　　处：《Transactions of Nanjing University of Aeronautics and Astronautics》2010年第1期51-54,共4页南京航空航天大学学报（英文版）

基　　金：Supported by the Natural Science Foundation of Jiangsu Province (07KJD14028)~~

摘　　要：The FLS900 steady-state fluorescence spectrometer is used to test the blood in vitro radiated by the continuous laser. Variation components in the blood and the fluorescence characteristic decay with the time are given. Results show that when the blood is excited by the laser of 407 nm, the location of the fluorescence peak is unchanged, and the intensity of the emission peak at 605 nm is slowly decreased. But the fluorescence spectrum wave changes after 14 h, and eventually has a strong fluorescence peak at 474 nm. It is shown that the laser can obviously put off the decay time of the blood in vitro. After the laser continuously irradiates for a period of time, it can lead to the changes of cell morphology and accelerates the hemolytic process. The blood cell decay produces many fluorescent substances-the reduction of the nicotinamide adenine dinucleotide. The result provides a valuable reference to study the activity of blood cells and the functional abnormalities of the blood.采用FLS900型稳态荧光光谱仪对激光持续照射的离体血液进行了荧光检测,得到了血液成分变化及荧光光谱特征随时间衰变的规律。实验结果表明:随着血液离体时间的延长,当受407 nm的光激励时,605 nm处的荧光峰峰位保持不变仅强度缓慢减弱。但在离体后的14 h出现了谱形突变,并在474 nm处产生了一个强荧光峰。分析认为,激光对离体血液的衰变有明显的延缓作用,但当激光持续照射一定时间后却导致了细胞的形态改变进而加速了细胞的溶血进程,产生了大量的内源性荧光物质还原烟碱腺嘌呤二核苷酸。研究结果为研究血细胞的活性衰变及细胞功能结构异常等提供了参考。

关 键 词：fluorescence spectroscopy LASERS blood cells 

分 类 号：O433.4[机械工程—光学工程]