反相高效液相色谱法同时测定四妙丸中盐酸小檗碱与苍术素的含量  

Simultaneous determination of berberine hydrochloride and atractylodin in Simiao pills by RP-HPLC

作　　者：张晓兰[1] 王晓玉[1] 张丽媛[1] 李利新[1] 李遇伯[1] 

机构地区：[1]天津中医药大学中药学院天津中药化学与分析重点实验室,天津300193

出　　处：《中南药学》2010年第4期259-262,共4页Central South Pharmacy

基　　金：国家自然科学基金(编号:30801540)

摘　　要：目的建立四妙丸中盐酸小檗碱和苍术素含量的测定方法。方法采用高效液相色谱法，色谱柱为Waters Symmetry C18（150mm×4．6mm，5μm）柱，流动相为乙腈0．1％H3PO4溶液，梯度洗脱，流速1．0mL·min^-1，检测波长340nm，柱温为20℃。结果被测峰与相邻峰可达到基线分离；盐酸小檗碱和苍术素的线性范围分别为6．25～125．00μg·mL^-1（r=0．9999）和8．00～160．00Mg·mL^-1（r=0．9997）；平均回收率（n=9）分别为99．2％、98．7％，RSD分别为2．12％、1．97％。结论本方法准确、简便、重复性好，可作为四妙丸中盐酸小檗碱和苍术素的含量测定方法。Objective To develop an RP HPLC method for the simultaneous determination of berberine hydrochlorideand atractylodin in Simiao pills. Methods The Waters Symmetry C18 column （150 mm×4.6mm,5μm） was used. The mobile phase consisted of acetonitrile-0.1% H3 PO4 solution at 1.0 mL ·min^-1. The detective wavelength was 340 nm and the column temperature was 20℃. Results The linear range of berberine hydrochloride and atractylodin was 6.25-125.00 （r=0. 999 9） and 8.00--160.00μg·mL^-1 （r=0. 999 7）, respectively. The average recoveries （n= 9） of the 2 components in Simiao pills was 99.2% and 98.7%, while RSD was 2. 12% and 1.97%, respectively. Conclusion The method is simple, precise and reliable for the determination of berberine hydrochloride and atractylodin in Simiao pills.

关 键 词：四妙丸 盐酸小檗碱 苍术素 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]