高效液相色谱法测定分析中小学男生CYP3A活性  

Study on CYP3A activity of school boys by HPLC

作　　者：刘海涛[1] 祝建[1] 陈同辛[2] 唐跃年[3] 张健[3] 

机构地区：[1]上海同济大学生命与技术学院,上海200092 [2]上海市儿科医学研究所免疫/肿瘤研究室,上海200090 [3]上海交通大学医学院附属新华医院药剂科,上海200092

出　　处：《实用药物与临床》2010年第2期122-123,共2页Practical Pharmacy and Clinical Remedies

基　　金：上海市卫生局课题项目(044057)

摘　　要：目的通过高效液相色谱法测定CYP3A酶活性,研究中小学男生CYP3A酶活性变化及其趋势。方法用高效液相色谱法梯度洗脱法测定并计算515名中小学男生尿液中6β-羟基氢化可的松和氢化可的松的比值,以反映CYP3A的活性。通过统计分析,研究CYP3A的活性的变化及其趋势。结果酶的活性在青春期前后都较平稳,但在开始发育时出现酶活性降低,有统计学意义,且发育后酶活性个体差异变大。结论中小学男生年龄跨越大,临床用经CYP3A酶代谢药物时,应根据身体发育情况适当调整。Objective To study the variety and trend of Cytochrome P450 3A（CYP3A）activity of school boys by HPLC.Methods The concentration of 6β-hydroxycortisol and cortisol in urinary were determined by HPLC,the variety and trend of CYP3A activity were analyzed by 6β-hydroxycortisol-cortisol ratio.Results The activity of the CYP3A fell and the coefficient of variation became larger when students began to come into adolescence.Conclusion The drugs metabolized by CYP3A should be properly adjusted according to developmental status of students.

关 键 词：6β-羟基氢化可的松 氢化可的松 CYP3A酶 活性 

分 类 号：R96[医药卫生—药理学]