旋毛虫肌蚴抗原诱导小鼠抗日本血吸虫保护性免疫的研究  

CROSS PROTECTION AGAINST SCHISTOSOMA JAPONICUM INFECTION IN MICE IMMUNIZED WITH TRICHNELLA SPIRALIS MUSCLE LARVA ANTIGEN *

作　　者：田明礼[1] 易新元[1] 曾宪芳[1] 曾庆仁[1] 彭先楚[1] 

机构地区：[1]湖南医科大学寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》1998年第6期411-414,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：湖南省科委资助

摘　　要：目的：探讨旋毛虫幼虫抗原免疫小鼠对日本血吸虫攻击感染的交叉保护作用。方法：用４种不同的旋毛虫幼虫抗原制剂经颈部皮下多点免疫小鼠，然后用日本血吸虫尾蚴３０条或１００条攻击感染，攻击后４５ｄ剖杀小鼠，收集成虫、肝组织和粪便中的虫卵，并计数。结果：４种不同制剂均可诱导不同程度的保护性免疫效应，以旋毛虫幼虫匀浆上清可溶性抗原（ＴｓＳＡ）效果较好，减虫率为２１．３％，加用福氏佐剂时，其减虫率为２９．３％，肝组织和粪便中的虫卵减少率分别达４８％和５８．５％，平均每鼠肝组织和粪便中的虫卵ＥＰＧ减少率分别为４１．７％和４８．９％；当抗原剂量为１００００条旋毛虫并加用福氏佐剂时，其减虫率达３９．６％。AIM: To explore the cross protection against S.japonicum cercaria challenge in mice immunized with T. spiralis larva antigen. METHODS:Groups of BALB/c mice were immunized with 4 preparations of T.spiralis larva antigen, respectively.Vaccinated mice were then challenged with 30 and 100 S.japonicum cercariae,respectively.Forty five days later,all mice were sacrificed and examined for adult worm burden,liver and stool egg count. RESULTS: All groups of mice immunized with 4 preparations of T.spiralis larvae antigen without adjuvant showed significant protection against S.japonicum . Among these antigen preparations,the soluble antigen (TsLSA) presented highest protection with reductions in worm burden and liver and stool egg counts of 21 3%, 48 0% and 58 5%, respectively.When mice were immunized with TsLSA plus Freunds complete adjuvant (FCA)or with double dose of the antigen,the worm reduction rate was increased to 29 3% and 39 6% , respectively. CONCLUSION:Different preparations of T. spiralis muscle larva antigen could induce protection against S.japonicum in mice.

关 键 词：旋毛虫 日本血吸虫 感染 免疫 交叉保护作用 

分 类 号：R383.15[医药卫生—医学寄生虫学] R383.24[医药卫生—基础医学]