新疆绵羊品种Ⅰ型IF基因的遗传多态性分析  

Polymorphism of TypeⅠIF Gene in Six Sheep Breeds

作　　者：曾献存[1] 贾斌[1] 赵宗胜[1] 王遵宝[1] 惠文巧[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2010年第2期177-179,共3页Journal of Shihezi University(Natural Science)

基　　金：国际科技合作项目(2007DFB30420)

摘　　要：为了筛选与绵羊羊毛性状相关联的分子标记,探讨Ⅰ型角蛋白中间丝蛋白基因多态位点在不同绵羊品种中的分布规律。采用PCR-RFLP技术对新疆6个绵羊品种Ⅰ型IF基因第1外显子的多态性进行了检测。结果显示,Ⅰ型IF基因第1外显子经MSPI酶切后产生2种等位基因和3种基因型,呈现多态。各绵羊品种之间基因型频率和等位基因频率差异不显著(P>0.05)。和田羊和巴音布鲁克羊群体在该位点上处于Hardy-Weinberg平衡状态(P>0.05)。平均He和PIC分别为0.2374和0.2079。To identify new DNA markers which have significant impact on sheep wool traits and the distribution pattern of polymorphic site of the type Ⅰ IF （intermediate filament wool keratin）gene in different sheep breeds. In this study, the polymorphism of the first exon of Type Ⅰ IF gene was detected by PCR- RFLP method in 6 local sheep breeds in Xinjiang. The results showed that two alleles （M=0. 8609 and N =0. 1391） and three genotypes （MM=0. 7776,MN=0. 1667 and NN=0. 0558） were detected across the 6 sheep breeds. Chi-square analysis indicated that the allele frequency and genotype frequency did not differ among the observed breeds （P〉0.05）. This polymorphic locus of Type Ⅰ IF gene was at Hardy-Weinberg equilibrium （P〉0.05） in the Hetian and Bayinbuluke sheep breeds. The average He and PIC were 0. 2374 and 0. 2079, respectively.

关 键 词：绵羊 Ⅰ型IF基因 多态性 

分 类 号：S826[农业科学—畜牧学]