血管内皮祖细胞促血管化组织工程骨修复兔大段骨缺损的效果评价  

Evaluation of repair of large segmental defects with vascularized tissue-engineered bone induced by endothelial progenitor cells in rabbits

作　　者：吴雪晖[1] 谢肇[1] 何清义[1] 许建中[1] 曾玲[1] 陈卫军[2] 孙东[1] 

机构地区：[1]第三军医大学附属西南医院骨科,重庆400038 [2]第三军医大学解剖学教研

出　　处：《中华创伤杂志》2010年第3期275-279,共5页Chinese Journal of Trauma

基　　金：国家高技术研究发展计划重大攻关课题（863计划）资助项目（2006AA02A122）;重庆市科委自然科学基金计划资助项目（CSTC,2007BB5044）

摘　　要：目的 评价血管内皮祖细胞（endothelial progenitor cells,EPCs）促血管化组织工程骨修复兔桡骨大段骨缺损的效果.方法 选用68只新西兰大耳白兔,以数字随机法分为3组.实验组：EPCs＋经成骨诱导的骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）＋脱钙骨基质（decalcified bone matrix,DBM）;自身对照组：BMSCs＋DBM;阴性对照组：单纯DBM.将上述材料置入桡骨中段15 mm骨缺损区,术后12,16周摄X线片行骨密度、组织学光镜、骨钙素免疫组化染色及生物力学测试.结果 实验组的骨痂生长、塑形、髓腔再通、骨愈合速度及力学强度等方面均明显优于对照组.结论 EPCs促血管化组织工程骨成骨能力强,能有效促进骨愈合,是修复大段骨缺损的有效方法.Objective To evaluate the bone healing effect of vascularized tissue-engineered bone induced by endothelial progenitor cells（EPCs）in repair of large segmental radius defects in rabbits.Methods A total of 68 healthy New Zealand white rabbits were enrolled in the study and randomized into three groups,ie,experimental group（EPCs group）：EPCs plus bone marrow mesenchymal stem cells （BMSCs）plus decalcified bone matrix（DBM）;control group：BMSCs plus DBM;sham control group：pure DBM.Materials mentioned above were implanted into middle radius defects for 15 mm.At 12 and 16 weeks post-operatively,X-ray test,bone mineral density test,histological light microscopic test,osteocalcin immunohistochemical staining test and biomechanical test were carried out.Results Growth and plasticity of callus,speed of medullary cavity recanalization,bone healing speed and biomechanical intensity in the experimental group were all significantly better than those of control group.Conclusions Vascularized tissue-engineered bone induced by EPCs has strong osteegenic ability,can accelerate bone healing and hence is an effective method for repair of large segmental bone defects.

关 键 词：组织工程 干细胞 骨折愈合 骨缺损 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]