结扎腹主动脉加双侧腹壁阴部动脉在大鼠后肢缺血模型中的应用  

Application of Abdominal Aortic and Bilateral Abdominal Perineal Artery Ligation in Hindlimb Ischemia Model of Rats

作　　者：刘坤[1] 杨琨[2] 罗军[1] 王洋[1] 李新喜[1] 白超[1] 揭月高[1] 

机构地区：[1]新疆医科大学第一附属医院消化血管外科中心,乌鲁木齐830054 [2]新疆医科大学第一附属医院烧伤整形科,乌鲁木齐830054

出　　处：《中国普外基础与临床杂志》2010年第3期258-262,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨结扎腹主动脉加双侧腹壁阴部动脉在SD大鼠后肢动脉缺血模型中的应用价值。方法将38只大鼠按随机数字表法随机分为假手术组(n=12)、结扎腹主动脉组(n=12)和结扎腹主动脉加双侧腹壁阴部动脉组(n=14)。氯胺酮6mg/100g腹腔注射麻醉大鼠,开腹后按各组不同的处理方法处理血管,观察术后2周和4周时各组后肢肌力、苍白等情况,同时取股静脉血做血气分析,并取后肢骨骼肌行HE染色。结果结扎腹主动脉加双侧腹壁阴部动脉组术后3d内死亡2只大鼠,其余大鼠均存活。假手术组术后无明显异常。结扎腹主动脉组术后立即出现后肢苍白、活动差;2周时后肢苍白、皮温低有好转;4周时恢复正常。结扎腹主动脉加双侧腹壁阴部动脉组术后2周时后肢缺血明显,肢体苍白、运动差;4周时仍有较明显的肌力异常、苍白等表现。各组均无后肢坏死。结扎腹主动脉加双侧腹壁阴部动脉组在术后2和4周时的肌力和苍白情况均较假手术组差(P<0.05);与结扎腹主动脉组比较,在术后4周时肢体苍白情况仍较差(P<0.05)。各组内不同时相间比较,差异均无统计学意义(P>0.05)。结扎腹主动脉加双侧腹壁阴部动脉组的静脉血氧分压在术后2及4周时明显低于其他2组(P<0.05)。HE染色:对照组显示正常的横纹肌结构;结扎腹主动脉组术后2周可见细胞染色不均,肿胀,横纹消失,部分细胞核染色深,4周时恢复正常;结扎腹主动脉加双侧腹壁阴部动脉组术后2周及4周见骨骼肌染色不均,细胞肿胀明显,横纹消失,部分细胞核染色深,密集,细胞间隙加大,但未见明显的细胞坏死。结论结扎腹主动脉加双侧腹壁阴部动脉可制作稳定的SD大鼠后肢缺血模型。Objective To investigate the application of abdominal aorta and bilateral abdominal perineal artery ligation in a hindlimb ischemia model of rats for research.Methods According to the random digits table,38 SD rats were divided into 3 groups randomly,sham operation group（SO group,n=12）,abdominal aorta ligation group（AAL group,n=12）and abdominal aorta and bilateral abdominal perineal artery ligation group（AAL＋BAPA group,n=14）.Rats were anesthetized by 6 mg/100 g ketamine via intraperitoneal injection.Blood vessels were ligated via laparotomy according to different procedures.Movement and pale skin color of rat＇s hindlimb were observed on 2 and 4 weeks after operation,meanwhile venous blood from unilateral iliac vein was obtained for blood gas analysis and hindlimb skeletal muscle for HE stain.Results Two rats were dead during 3 d after operation in AAL＋BAPA group,other rats survived.Rats in SO group had no obviously abnormal appearance.AAL group and AAL＋BAPA group immediately presented hindlimbs pale,lower skin temperature,hypofrontality of limb motion after procedure.Symptoms above mentioned had improved gradually after 2 weeks and completely recuperated 4 weeks after operation in AAL group.Ischemia symptoms were still remained obviously such as cold,dried and thin on the 4th weekend in AAL＋BAPA group.Each group had no hindlimbs necrosis.Two weeks after operation,pale limbs and muscle strength in AAL＋BAPA group were more severe than those of SO group（P0.05）;Pale limbs was still worse than that of AAL group on 4 weeks after operation（P0.05）.There were no significant differences on different time in each group（P0.05）.Venous blood partial pressure of oxygen of AAL＋PABA group was significantly lower than that of the other two groups on 2 and 4 weeks after operation（P0.05）.Normal striated muscle structure was presented in SO group pathologically.AAL group revealed coloretur unevenness,swelling and distension,muscle cellular transverse striation elimination,skeletal muscle cell

关 键 词：肢体缺血 腹主动脉 腹壁阴部动脉 结扎 动物模型 

分 类 号：R654.3[医药卫生—外科学] R364.1[医药卫生—临床医学]