NS-398诱导尖锐湿疣患者外周血PBMC凋亡及对调控蛋白表达的影响  

The impact of cyclooxygenase-2 inhibitor NS-398 on apoptosis and expression of regulative protein of peripheral blood mononulear cells in condyloma acuminatum

作　　者：徐超[1] 栗玉珍[1] 

机构地区：[1]哈尔滨医科大学附属第二临床医学院皮肤科,150086

出　　处：《中国麻风皮肤病杂志》2010年第3期161-163,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:评价环氧化酶2(COX-2)抑制剂NS-398对尖锐湿疣患者外周血单个核细胞(PBMC)凋亡及凋亡调控蛋白表达的影响。方法:采用流式细胞仪检测不同浓度NS-398作用CA患者PBMC24 h后细胞凋亡及凋亡调控蛋白的表达。结果:100-200μmol/L的NS-398对PBMC具有明显诱导凋亡的作用,随NS-398浓度增加,凋亡调控蛋白Apo-1、Apo-2.7表达水平呈下降趋势,Bcl-2表达水平呈上升趋势。结论:NS-398可以通过抑制COX-2的途径促进PBMC凋亡,使PBMC的凋亡调控失衡得以恢复。Objective： To investigate the effect of cyclooxygenase - 2 inhibitor NS - 398 on the apoptosis and regulative protein expression of peripheral blood mononuclear cells （PBMCs） in condyloma acuminatum （CA）. Methotis： Apoptosis and expression of regulative protein of PBMC cultured in different concentrations of NS - 398 medium for 24 h were detected by flow cytometry. Results： The apoptosis of PBMC cultured in 100 and 200 μmol/L NS - 398 medium for 24 h was increased. As the increase of concentrations of NS - 398, the expressions of regulative protein Ape - 1 and Ape - 2.7 were decreased. But the Bcl - 2 expression continued to increase. Conclusion： NS - 398 can induce apoptosis of PBMC and restore uncontroled apoptosis balance of PBMC.

关 键 词：PBMC 环氧化酶抑制剂 细胞凋亡 凋亡调控蛋白 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]