溶胶-凝胶生物活性玻璃/胶原复合多孔支架的力学性能研究  

Investigation on mechanical property of the compound scaffold of Sol-Gel bioactive glass/collegan

作　　者：张娟娟[1,2] 孟永春[1,2] 陈晓峰[1,2] 李玉莉[1,2] 罗小刚[1,2] 林才[1,3] 

机构地区：[1]华南理工大学生物医学工程研究院,广州510640 [2]华南理工大学特种功能材料教育部重点实验室,广州510640 [3]温州医学院附属第一医院

出　　处：《医用生物力学》2010年第1期16-20,25,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金(50672028);国家自然科学基金重点项目(50830101;50732003);浙江省自然科学基金(Y407241)

摘　　要：目的研究溶胶-凝胶生物活性玻璃/胶原组织工程支架的力学性能及降解性能,为胶原基复合支架的进一步应用,提供理论基础。方法以纳米溶胶-凝胶生物活性玻璃为添加相,利用冷冻干燥法制备了4种溶胶-凝胶生物活性玻璃/胶原基复合多孔组织工程支架。结果(1)溶液中胶原纤维的聚集状态,制备出具有直径约为400～600nm的粗胶原纤维束支架材料,这种粗胶原纤维束对改善胶原基组织工程支架的力学强度和减慢其降解速度具有重要作用,其中胶原与生物活性玻璃质量比为40∶60时,具有最高的抗压强度(1.5469±0.0995)MPa。(2)利用FTIR和Raman等技术综合分析研究了溶胶-凝胶生物活性玻璃对胶原蛋白的二级结构的影响,当复合材料中胶原含量小于20%时,胶原蛋白二级结构破坏严重。结论当胶原与生物活性玻璃质量比为40∶60时,所制备的复合支架具有最好的抗压性能和降解性能,为进一步应用提供了研究基础。Objective To investigate the mechanical prosperity and degradation rate of the scaffolds with compounding collagen and the nano sol-gel derived bioactive glass were studied,and provide the theoretical basis for the further application of collagen based scaffolds.Method The scaffold with compounding collagen and the nano sol-gel derived bioactive glass（58S）were prepared using the freeze-drying techniques with the bioactive glass as phase addition.By affecting the aggregation state of the collagen fibers with adjusting the supplementation of bioactive glass to change the microstructures of the compound scaffolds and finally the compound scaffolds with different mechanical properties were prepared.Results（1）As the aggregation state of the collagen fibers changes,the scaffolds with the coarser collagen fibers is prepared with the diameters 400-600 nm approximately.The coarser collagen fibers will play an important role in improving the mechanical property and slowing down the degradation rate of the collagen based scaffolds.（2）The interactions between bioactive glass and collagen are studied by FTIR and Raman technologies.When the quality of content of collagen in the compound scaffolds is lower than 20%,the secondary structure of collagen is damaged severely.Concluslon The composite scaffolds with the mass ratio of collagen to bioactive glass 40∶60 has the best performance in mechanical property and degradation,which will be helpful for further applications.

关 键 词：胶原 生物活性玻璃 支架 力学性能 降解 

分 类 号：R318.01[医药卫生—生物医学工程]