苯并[a]芘降解菌SL-1的筛选、鉴定及其降解特性  

LSOLATION,IDENTIFICATION AND DEGRADATION PROPERTIES OF A BENZO[a]PYRENE-DEGRADING STRAIN SL-1

作　　者：李艳秋 刘君 高鹏 刘慧军 孙建光[2] 赵吉[3] 胡海燕[2] 屹立 

机构地区：[1]内蒙古煤炭建设生态环境研究院,呼和浩特010020 [2]中国农业科学院资源区划所,北京100081 [3]内蒙古大学生命科学学院,呼和浩特010018

出　　处：《环境与发展》2010年第2期74-77,共4页Environment & Development

基　　金：内蒙古自然科学基金(200508010604)

摘　　要：从土壤中筛选出一株以苯并[a]芘为唯一碳源的高效降解菌SL-1,经形态观察、生理生化分析以及16S rDNA基因序列分析,该菌株鉴定为假单胞菌(Pseudomonas sp.)。28℃震荡培养10 d后,菌株SL-1对5mg.L-1苯并[a]芘的降解率为35.7%;同时测定该降解菌的生长曲线、降解率变化曲线、最佳培养条件;土壤修复试验表明,42d时,降解菌SL-1对苯并芘的降解率达到76.3%。A bacterium strain SL - 1 was isolated from. soil by repeated subculture in liquid medium with benzo [a] pyrene as sole earbon source. To confirm the classification of SL - 1, morphological, physiological and biochemical characterization were applied and PCR was carried out to measure the gone sequence of 16S rDNA. The 16S rDNA was compared with related bacterias in GenBank, and phylogenetic tree was constructed. Based on the above results, the strain SL - 1 was identified as Pseudomonas. sp.. In a mineral salts medium with 5 mg . L - 1 benzo [a] pyrene, the highest degradation rate of 35.7% was obtained after 10 days at 28 ℃ ; and varied curves of growth, degradation rate,optimal conditions of growth. Experiments for bioremediation of bcnzopyrene - contaminated soil were detected, Within 42 days, the degrading rates were 76.3% respectively.

关 键 词：苯并[A]芘 降解菌 筛选 鉴定 

分 类 号：Q938.13[生物学—微生物学]