HPLC测定7种龙胆科植物花中龙胆苦苷与獐牙菜苦苷的含量  

Determination of Swertiamarin and Gentiopicroside in the Flowers of Seven Gentianaceae

作　　者：张兴旺[1,2] 陶燕铎[1] 梅丽娟[1] 邵赟[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]中国科学院研究生院,北京100049

出　　处：《中国野生植物资源》2010年第2期38-40,共3页Chinese Wild Plant Resources

基　　金：科技部国家科技支撑计划(2007BAI45B00)

摘　　要：目的：建立7种龙胆花中龙胆苦苷和獐牙菜苦苷含量测定的HPLC方法。方法：采用微波辅助动态回流法进行提取，色谱条件：Fusion—RP80A C18柱（150mm×4．6mm，5um）；流动相：甲醇-0．2％磷酸溶液梯度洗脱（0—25min：15％～30％）；流速：1mL／min；柱温：30℃；检测波长240nm。结果：7种龙胆花中獐牙菜苦苷和龙胆苦苷的色谱峰与共存组分完全达到基线分离，线性范围分别为0．105～0．945ug（r=0．9999），0．3—0．7ug（r=0．9999），平均加样回收率分别为97．8％（RSD=1．02％），98．9％（RSD=1．51％）。结论：所建立的方法测定快速，结果准确可靠。Objective： To establish a HPLC method for the determination of swertiamarin and gentiopicroside in the flowers of seven gentianaceae. Methods： A new method--microwave - assisted reflux with magnetic stir extraction was used. Experiments were performed on Fusion - RP 80 A C18（ 150 mm × 4.6 mm, 5um） column , with the mobile phase consisting of methanol and 0.2 % phosphoric acid at a flow rate of 1 mL/min. The operation was conducted in the gradient mode in which the ratio of methanol to 0.2% phosphoric acid varied from 15：85 to 30：70 in 25 min. The column temperature was 30 ℃ and the determination wavelength was 240 nm. Results： Chromatographic separation of swertiamarin, gentiop- icroside and the co - existed compounds achieved baseline resolution. The linear range were 0. 105- 0. 945 ug（r =0.999 9） and 0.3 -0.7（r =0. 999 9） separately, the average recovery were respectively 97.8% （RSD = 1.02% ） and 98.9% （RSD = 1.51% ）. Conclusion： The method is rapid and precise.

关 键 词：高效液相色谱 龙胆科植物花 獐牙菜苦苷 龙胆苦苷 

分 类 号：S567[农业科学—中草药栽培]