大豆疫霉根腐病菌的分离鉴定及种质资源对3号生理小种的抗性评价  

Isolation of Phytophthora sojae and Identification of Resistance to Race 3 in Soybean Germplasm

作　　者：徐鹏飞[1] 吴俊江[2] 范素杰[1] 陈晨[1] 李宁辉[1] 王金生[2] 李文滨[1] 张淑珍[1] 

机构地区：[1]东北农业大学大豆研究所,国家教育部大豆生物学重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086

出　　处：《大豆科学》2010年第2期272-275,共4页Soybean Science

基　　金：国家自然科学基金资助项目(30671317,30810103063,30400285);黑龙江省新世纪人才培养计划资助项目(NCET-06-007);黑龙江省自然科学基金资助项目(C200814);黑龙江省农业科学院博士后基金资助项目(LRB06-010);中国博士后基金资助项目(20060400835);博士后落户黑龙江科研启动基金资助项目(LBH-Q05032)

摘　　要：采用下胚轴伤口接种法,用在黑龙江省建三江农场分离到的大豆疫霉菌3号生理小种对292份栽培大豆材料(其中农家品种153份、其它大豆栽培品种139份)和236份野生大豆材料进行了抗性鉴定。结果表明:栽培大豆资源抗病80份,占27.4%,中间类型93份,占31.8%,感病119份,占40.8%。153份农家品种中,抗病的有49份,占农家品种的32.0%,表明农家大豆品种资源抗性比例较高。野生大豆资源中抗病的有49份,占20.8%;中间类型55份,占23.3%;感病132份,占55.9%。鉴定的这些高抗资源可为我国大豆抗疫霉根腐病育种奠定基础。Phytophthora root and stein rot of soybean caused by Phytophthora sojae is a destructive disease in soybean production regions all around the world. Utilization resistant cultivar is the most economical and environmentally safe method for preventing against the disease, and screening on resistance germplasm is the basement for resistance breeding. A total of 292 soybean cultivars （including 153 native soybean germplasm and 139 soybean cuhivars） and 236 wild soybean germplasm were inoculated with race 3 of Phtophthora sojae isolated from Jiansanjiang where there is serious disease occurring in Heilongjiang Province. A total of 80 cuhivars were resistant, 93 had intermediate reaction, and 119 were susceptible to race 3, representing 27.4% , 31.8% and 40.8% of all the cultivars tested respectively. The number of resistant native soybean was 49, representing 32.0% of all the native soybean germplasm, which means that the percentage of native resistant germplasm was higher. A total of 49 wild soybean germplasm were resistant, 55 had intermediate reaction, and 132 were susceptible to race 3, representing 20.8% , 23.3% , and 55.9% of all the wild soybean germplasm tested, respectively. These resistant germplasm can be used in breeding for resistance to P. sojae.

关 键 词：大豆种质资源 大豆疫霉根腐病 3号生理小种 抗性鉴定 

分 类 号：S565.1[农业科学—作物学]