熟地黄对弗氏佐剂诱导大鼠足趾炎症的影响  

Effect of Radix Rehmanniae Praeparata on footpad inflammation induced by complete Freund's adjuvant in Wistar rats

作　　者：王雪燕[1] 温学森[1] 张迪[1] 孔晓妮[1] 史敏[1] 

机构地区：[1]山东大学药学院生药学研究所,山东济南250012

出　　处：《中国生化药物杂志》2010年第2期103-106,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金项目(30472189);山东省自然科学基金资助项目(2009ZRB01916)

摘　　要：目的熟地黄常出现于治疗类风湿性关节炎的方剂中,本文用完全弗氏佐剂诱导大鼠足趾炎症,旨在验证其是否具有抗炎作用。方法于大鼠右后足趾皮内注射完全弗氏佐剂100μL。致炎后第19天,灌胃给予高、中、低剂量的熟地黄[0.625,1.250,2.500g/(kg.体重)],10d,以地塞米松为阳性药物,记录大鼠体重和足趾体积变化;第29天,测定脏器、血液参数,取足趾肉垫切片观察。结果模型组大鼠足趾持续肿胀,淋巴细胞浸润,胸腺和脾脏指数下降,血小板显著减少,其它指标与对照组差异不显著。熟地黄未显示抗炎作用,但可使胸腺和脾脏指数,以及血小板计数恢复正常,使肌酐水平显著上升。地塞米松抗炎作用显著,但同时存在多项毒副作用。结论熟地黄在治疗类风湿性关节炎的方剂中主要发挥辅助作用。Purpose To investigate the anti-inflammatory effect of Radix Rehmanniae Praeparata （RRP） on the footpad inflammation induced by complete Freund＇s adjuvant （CFA） in rats.Methods CFA 100 μL were injected subcutaneously to the Wistar rats at the pad of right hindfoots.19 days later,the rats were daily given RRP water extract （0.625,1.250,2.500 g/kg·bw） or dexamethasone （0.5 mg/kg·bw） intragastrically.The changes of body weight and foot volume were measured.The indexes of organ and blood were determined at the 29th day,the foot pad was removed,and routine paraffin section was performed.Results The model rats kept foot swelling and lymphocyte infiltrating,and the platelet number decreased.The other indexes were statistically insignificant when compared to the controls.RRP did not display any anti-inflammatory effect on the swollon foots,but thoracic gland and spleen indexes were rescued,and platelet number and creatinine content were increased by RRP administration in a dose-dependent manner.The anti-inflammation of dexamethasone was conspicuous,but the side effects were also significant.Conclusion RRP may be plays an adjunctive action in herbal recipes to treat rheumatoid arthritis.

关 键 词：熟地黄 完全弗氏佐剂 类风湿性关节炎 地塞米松 

分 类 号：R285[医药卫生—中药学]