前胡甲素对小鼠局灶性脑缺血的保护作用  

Protective effect of dl-praeruptorin A on focal cerebral ischemia in mice

作　　者：杨武双[1] 滕伯刚[2] 杨立朝[2] 周宇[2] 王瑶[2] 金鑫[2] 

机构地区：[1]厦门市中医院神经外科,福建厦门361009 [2]厦门大学医学院药学系,福建厦门361005

出　　处：《中国生化药物杂志》2010年第2期118-121,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的观察前胡甲素(Pd-Ia)对小鼠局灶性脑缺血损伤的保护作用及特点。方法线栓法制备小鼠大脑中动脉栓塞脑缺血损伤模型。Pd-Ia(1,5,10mg/kg)在缺血前0.5h腹腔给药1次;或在缺血前1,0.5h、缺血同时、再灌注同时、再灌后0.5h及再灌后1h各腹腔给予Pd-Ia5mg/kg。脑缺血1.5h,再灌注24h后,测定小鼠神经功能缺失评分、脑梗死体积、脑水肿等评定脑缺血损伤的指标;测定血清中丙二醛(MDA)和超氧化物岐化酶(SOD)的活性。结果Pd-Ia(5,10mg/kg)缺血前0.5h给药及Pd-Ia5mg/kg缺血前0.5h、缺血同时、再灌注同时及再灌后0.5h给药可明显改善小鼠神经功能损伤,减小脑梗死体积和减轻脑水肿程度,且以再灌注同时单次给药效果最为显著;Pd-Ia(5,10mg/kg)能够明显提高脑缺血损伤小鼠血清中SOD活性,降低MDA含量。结论Pd-Ia保护小鼠局灶性脑缺血急性损伤,最佳剂量为5mg/kg,最佳治疗时间点为再灌注同时;其保护脑缺血损伤的机制可能与抑制脂质过氧化、提高氧化酶的活性有关。Purpose To investigate the protective effect and character of dl-praeruptorin A （Pd-Ia） on focal cerebral ischemia in mice.Methods Transient focal cerebral ischemia in mice was induced by middle cerebral artery occlusion for 1.5 h.Pd-Ia was administered intraperitoneally either with multiple doses （1,5 and 10 mg/kg） at 0.5 h before ischemia or single dose （5 mg/kg） at 0.5 h and 1 h before ischemic,the same time of ischemia,the same time of reperfusion,or 0.5 h and 1 h after reperfusion respectively.Neurological deficit score,infarct volume,brain edema,the activities of SOD and the contents of MDA were determined.Results Pretreatment with multiple doses （5 and 10 mg/kg） of Pd-Ia at 0.5 h before ischemia or single dose （5 mg/kg） of Pd-Ia at 0.5 h before ischemia,at the same time of ischemic,at the same time of reperfusion and 0.5 h after reperfusion significantly attenuated neurological deficit score,decreased infarct volume and alleviated brain edema,and the treatment at the time of reperfusion had the most marked effect.Pd-Ia （5 or 10 mg/kg） can significantly enhance the activities of SOD and lower the contents MDA.Conclusion dl-praeruptorin A has a neuroprotective effect on the injury in the acute phase of transient focal cerebral ischemia in mice,with optimal doses of 5 mg/kg and the optimal therapeutic time point of the same time of reperfusion.

关 键 词：前胡甲素 大脑中动脉栓塞 脑缺血 

分 类 号：R965[医药卫生—药理学]