六种TSH检测方法的对比研究  

Evaluation of Six Different Immunoassays for Serum Thyrotropin

作　　者：马冬红[1] 陆汉魁[1] 高云朝[1] 葛雯莉[1] 熊江[1] 刘俏平[1] 顾青[1] 

机构地区：[1]上海交通大学附属第六人民医院核医学科,上海200233

出　　处：《标记免疫分析与临床》2010年第2期102-106,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：采用六种免疫检测方法检测血清促甲状腺激素（TSH），分析检测方法间的差异性和相关性及对临床诊断的影响。纳入研究的样品150份，甲亢、甲减和表面健康人各50份。免疫检测方法包括一种放射免疫分析法（RIA），一种免疫放射分析法（MRIA），四种自动化检测方法（三种化学发光免疫分析法和一种电化学发光免疫分析法）。结果表明，六种免疫方法在检测血清TSH时相互之间存在不同程度差异（P〈0．05），其中RIA法在甲亢组和表面健康组的检测值与其他方法的检测值间均不具有相关性（P〉0．05）；IRMA法在甲亢组的检测值与四种自动化检测法的检测值相关系数较低（r为0．38—0．41）；四种自动化检测法两两之间的检测值问均高度相关（r为0．92—0．99）。在临床诊断符合率方面，四种自动化检测法相互间无显著差异；IRMA法在甲亢组同四种自动化检测法有显著差异（P〈0．01），而RIA法不能将甲亢病人同表面健康组区分，不宜用于甲亢诊断。To analyzes the discrepancy and association among six different thyrotropin （TSH） immunoassay methods and to study their impact on the clinical diagnoses of thyroid diseases, the 150 serum samples from three groups consisting of hyperthyroidism, hypothyroidism and healthy subjects, 50 samples in each group were includ- ed in this study. The serum TSH levels were measured simultaneously by radioimmunoassay （RIA）, immunoradiometric assay （IRMA）, three-type chemilumiminescence immunoassay （CLIA） and electrochemiluminescence immunoassay （ECLIA）. The results showed that individual serum TSH level varied significantly from one assay to another. There was no correlation between TSH RIA and other five assays in groups of hyperthyroidism and healthy subjects（P 〉 0.05 ）. The correlations between TSH IRMA and four automatic assays in hyperthyroidism group were relatively low（r = 0.38 -0.41 ）. However, among the four automatic assays, TSH levels were well correlated （r = 0.92 - 0.99）. For clinical diagnoses , TSH RIA alone was not useful in the differentiation of hy- perthyroidism and normal subjects, and TSH IRMA was misl nificant differences for four TSH automatic immunoassays in eading in some hyperthyroidism. There were no sigdifferential diagnoses of thyroid diseases.

关 键 词：自动检测 标记免疫分析法 促甲状腺激素 可比性 

分 类 号：R446.6[医药卫生—诊断学] R817.4[医药卫生—临床医学]