中毒事件中有机磷农药快速检测方法研究  

Rapid Detection of Organophosphorus Pesticide in Poisoning Incidents

作　　者：刘菁[1] 邵华[1] 许光[1] 张梦萍[1] 邹薇[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南市250062

出　　处：《职业与健康》2010年第8期845-847,共3页Occupation and Health

基　　金：国家公益性行业科研专项项目:化学中毒检测技术研究及平台建设;项目编号200802017

摘　　要：目的建立适用于中毒事件的有机磷农药的快速定性与半定量检测方法。方法在优化实验条件的基础上,样品用二氯甲烷超声萃取,有机磷农药在碱性条件下与4-(4-硝基苄基)吡啶反应,生成紫色化合物,550nm波长下用分光光度法测定含量。结果样品中毒死蜱的最低检出浓度为0.6mg/kg,回收率为85.8%～92.6%,相对标准偏差(RSD)为5.5%～8.7%。有机磷类农药均能与4-(4-硝基苄基)吡啶发生显色反应,而氨基甲酸酯类、拟除虫菊酯类、有机氯农药对测定无干扰。结论该方法快速、操作简便,可用于中毒事件中有机磷农药总量的快速定性与半定量检测。[Objective] To establish a method for rapid qualitative and semiquantitative determination of organophosphorus pesticides in poisoning incidents.[Methods]Samples were extracted with dichloromethane by the application of ultrasound, and then organophosphorus pesticide was reacted with 4-（4-Nitrobenzyl）pyridine （NBP） to generate purple compounds under optimal conditions. Then the purple compounds were detected by spectrophotometer at 550 nm wavelength.[Results]The detection limit of chlorpyrifos was 0.6 mg/kg;, the recoveries were 85.8%~92.6% and the relative standard deviation （RSD） ranged from 5.5% to 8.7%. All organophosphorus pesticides could react with NBP in color reaction, and pesticides of carbamates, pyrethroids, organochlorine caused no interference.[Conclusion]This method is simple, easy and suitable for the rapid qualitative and semiquantitative determination of total organophosphorus pesticides in poisoning incidents.

关 键 词：中毒 有机磷农药 快速检测 分光光度法 

分 类 号：R115[医药卫生—公共卫生与预防医学]