胚胎与成人骨髓CD34^+细胞RUNX1相关基因及Notch信号通路的比较研究  

Comparative study on RUNX1 genes and Notch signals of CD34^+ cells derived from human embryo and adult bone marrow

作　　者：李俊琴[1] 梁宏刚[1] 侯瑞霞[1] 尹国华[1] 张静[2] 张开明[1] 

机构地区：[1]太原市中心医院皮肤科,太原030009 [2]太原市中心医院内科,太原030009

出　　处：《山西医科大学学报》2010年第4期308-311,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30771940);山西省自然科学基金资助项目(2009011053-2)

摘　　要：目的了解引产儿胚胎与成人骨髓CD34+细胞生物学活性,寻找新的造血干细胞来源RUNX1相关基因和Notch信号通路差异。方法RT-PCR检测引产儿胚胎和成人骨髓CD34+细胞RUNX1、Hes-1、SLC9A3R1、Notch1、Notch2、HLA-C、PD-CD1、PKC-βI的mRNA表达水平。结果引产儿胚胎骨髓CD34+细胞RUNX1、Hes-1、SLC9A3R1、Notch1、Notch2、HLA-C、PDCD1和PKC-βI的mRNA表达水平与成人组比较差异均无统计学意义(P>0.05)。结论引产儿胚胎骨髓CD34+细胞具有与成人骨髓CD34+细胞相似的生物学活性,可以作为实验中正常对照使用,满足实验需要。Objective To explore the difference of RUNX1 genes and Notch signals in embryo and adult bone marrow derived CD34＋ cells.Methods The mRNA expression of RUNX1,Hes-1,SLC9A3R1,Notch1,Notch2,HLA-C,PDCD1 and PKC-βI was detected using reverse transcriptase-polymerase chain reaction（RT-PCR）.Results The expression of RUNX1,Hes-1,SLC9A3R1,Notch1,Notch2,HLA-C,PDCD1 and PKC-βI in bone marrow CD34＋ cells from human embryo and adult showed no significant difference（P0.05）.Conclusion The biological activity of CD34＋ cells from embryo bone marrow is similar to those from adult,and can be used as normal controls in experiments.

关 键 词：CD34+细胞 胚胎 成人 骨髓 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]