桑叶总生物碱中1-脱氧野尻霉素的测定及方法学考察  

Study on the Measurement and Methodology of 1-deoxynojirimycin in Total Alkaloids from Mulberry Leaf

作　　者：刘韦鋆[1] 赵骏[1] 曾森[1] 刘美平[1] 

机构地区：[1]天津中医药大学,研究生天津300193

出　　处：《天津中医药》2010年第2期166-168,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：高等学校科技创新工程重大项目培育基金项目资助课题(706013)

摘　　要：[目的]为提取纯化后的桑叶总生物碱供试品中的1-脱氧野尻霉素建立测定方法及对方法学进行考察。[方法]1-脱氧野尻霉素供试品的含量测定采用柱前衍生化高效液相色谱法。[结果]制桑叶总生物碱供试品3份,经测定,供试品中1-脱氧野尻霉素的含量分别为7.99%,8.64%,7.09%。[结论]检测方法简单、快速、准确。[Objective] To extract and purify 1-deoxynojirimycin in total alkaloids from mulberry leaf and to establish the method of eontent determination. [Methods] The 1-deoxynojirimycin in total alkaloids from mulberry leaf was measured by using pre-column derivation high performance liquid chromatography. [Results] In this way, the purity was exceeded above 7.99%, 8.64%, 7.09%. [Conclusion] This method was simple, high-speed, and accurate.

关 键 词：桑叶 1-脱氧野尻霉素 柱前衍生化 

分 类 号：R917[医药卫生—药物分析学]